β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit
Invitrogen™

β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit

β-hydroxybutyrate (β-HB), C4H8O3, accounts for about 75% of the total ketone body. Patients with diabetic ketoacidosis have increased production of NADH, which promotes the conversion of acetoacetic acid into β-HB. Therefore, the level of β-hydroxybutyrate can be used as an index to evaluate glycosuria.
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货号数量
EEA04696 Tests
货号 EEA046
价格(CNY)
7,120.00
Each
数量:
96 Tests
价格(CNY)
7,120.00
Each

With this kit, β-hydroxybutyrate dehydrogenase catalyzes the oxidative dehydrogenation of β-HB. Meanwhile, NAD+ is reduced to NADH, which under the action of hydrogen transmitter, transfers electrons to WST-8 to produce a yellow product. The content of β-HB can be calculated by measuring the change of absorbance value at 450 nm. This kit is useful for researchers studying ketone body metabolism and diabetes.

Key features

  • Simple and fast protocol—user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
  • Broad range of kits across key analytes and targets within the most important metabolic pathways
  • Consistent—strict quality control criteria to ensure reproducible and reliable results
For Research Use Only.
规格
测定范围0.01 to 2.00 mmol/L
检测灵敏度0.01 mmol/L
适用于(设备)Microtiter Plate Reader
孵育时间40 min.
检测间 CV4.0%
检测内 CV2.0%
产品类型Kit
数量96 Tests
样品体积10 μL
运输条件Dry Ice, 2°C to 8°C
指定目标试剂盒β-Hydroxybutyrate (Ketone Body)
总测定时间60 min.
检测方法Colorimetric
免疫测定试剂盒格式Colorimetric Detection Kit
样品类型Serum, Plasma, Urine, Animal Tissue
目标种属Universal
Unit SizeEach
内容与储存
Store at 2°C to 8°C.