TheraPure™ SuperFi™ II DNA Polymerase

TheraPure SuperFi II DNA Polymerase is a high-fidelity, proofreading DNA polymerase that combines exceptional accuracy with an optimized buffer system enabling a universal primer annealing temperature. Manufactured using animal-origin-free (AOF) and beta-lactam-free processes, it supports nucleic acid-based therapeutic development workflows.

Key Features:

• Animal-origin-free and beta-lactam-free manufacturing: Produced using processes and materials free from animal-derived components and beta-lactam compounds, supporting sensitive applications
• exceptional fidelity performance: Delivers >300-fold higher fidelity than standard Taq polymerase for accurate DNA amplification
• Extended amplification range: Enables long-range PCR of DNA targets up to 20 kb, suitable for large genomic regions and complex templates
• Standardized primer annealing temperature: Universal 60°C annealing temperature simplifies PCR optimization and enables consistent thermal cycling across primer sets

TheraPure SuperFi II DNA Polymerase incorporates Affibody-based hot-start technology to inhibit enzyme activity at ambient temperatures, allowing room-temperature reaction setup and extended stability of pre-assembled PCR reactions. Enzyme activity is restored during the initial denaturation step enabling high specificity.

TheraPure™ 5X SuperFi™ II Buffer contains specialized additives that support efficient amplification of both AT-rich and GC-rich templates. Robust performance is achieved with GC-rich sequences up to 75% GC without the need for additional melting agents. Buffer additives stabilize primer–template duplexes during annealing, enabling increased specificity without individual primer optimization.

TheraPure SuperFi II DNA Polymerase possesses 5'→3' polymerase and 3'→5' exonuclease (proofreading) activities and lacks 5'→3' exonuclease activity. The enzyme generates blunt-end DNA products.