FilmTracer™ FM™ 1-43 Green 生物膜细胞染色剂

FM lipophilic styryl dyes are known to shift their spectral properties upon binding to membranes. These dyes exhibit an appreciable solvent and environmental dependency in both free and membrane-bound forms. The emissions of the membrane-bound dye shifts to a shorter wavelength compared to the emission in the solvent.

In solvent, FM 1-43 Dye (Cat. Nos. T35356, T3163, F35355, F10317) absorbs in the range of fluorescein (~490 nm) and initially emits at around 626-636 nm. However, upon membrane integration, it exhibits emission in the 565-590 nm range. Because this dye exhibits a large Stokes shift, it does not perfectly fit into a green or orange fluorophore profile, but is rather a yellow-fluorescent dye, and may require a long-pass emission filter.

Note: FM 1-43 Dye is often used with typical fluorescein or GFP optical filter sets for biological imaging but is poorly excited through tetramethylrhodamine filters.

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