Fura-2, AM，细胞渗透性（无水 DMSO 中的1 mM 溶液）
Fura-2, AM，细胞渗透性（无水 DMSO 中的1 mM 溶液）
Invitrogen™

Fura-2, AM，细胞渗透性（无水 DMSO 中的1 mM 溶液）

Fura-2 AM 是一种高亲和力细胞内钙指示剂，其可进行比率测量且可被紫外光激发。这种乙酰氧基甲基 (AM) 酯形式适用于无创的细胞内上样，可以 1 mg 含量 (F-1201)了解更多信息
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货号数量
F12251 mL
货号 F1225
价格（CNY)
5,065.00
Each
添加至购物车
数量:
1 mL
价格（CNY)
5,065.00
Each
添加至购物车
Fura-2 AM 是一种高亲和力细胞内钙指示剂，其可进行比率测量且可被紫外光激发。这种乙酰氧基甲基 (AM) 酯形式适用于无创的细胞内上样，可以 1 mg 含量 (F-1201) 形式提供也可用于特殊包装 (F-1221)。可提供细胞不可透过性五钾 (F-1200) 和五钠 (F-6799) 盐形式的染料。

了解更多有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的信息›

仅供科研使用。不可用于诊断程序。
规格
最大浓度1 mM
检测方法荧光
染料类型基于荧光染料
数量1 mL
运输条件室温
适用于（应用）细胞活力与增殖
适用于（设备）荧光显微镜
产品类型Fura-2 AM
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.