Fluorimetric quantification of cell death in monolayer cultures and cell suspensions.
Authors:Ruiz MC, Michelangeli F, Ludert JE, Liprandi F, del Castillo JR, Chemello ME, Benaim G, Cohen E
Journal:J Biochem Biophys Methods
PubMed ID:1779095
'A fluorimetric assay using ethidium bromide (EB) was employed to quantify cell death in monolayer cell cultures (MA-104 cells) in situ and isolated cell suspensions (isolated colonic cells and Leishmania). Fluorescence of EB stained cells was measured with a photometer coupled to an inverted microscope for cell monolayers or in ... More