包含: • 200 μL Phire 热启动 II DNA 聚合酶 • 5 x 1.25 mL 2X Phire 动物组织 PCR 缓冲液(含 dNTP 和 MgCl2) • 40 μL 对照引物混合物(各 25 μM) • 2 x 5 mL 稀释缓冲液 • 3 x 100 μL DNARelease 添加剂 • 3 x 1 mL 凝胶上样染料
在 -20°C 下储存。
常见问题解答 (FAQ)
What is your recommendation to take a sample from plant or animal tissue for Direct PCR?
In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.
Why are Control primers provided in the Direct PCR Kits?
It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.
How are samples, stored at different conditions, compatible with the Phire Tissue Direct PCR Master Mix?
With the Phire Tissue Direct PCR Master Mix you can amplify DNA from differently stored tissues (fresh, frozen, FFPE) from human and a variety of animal species.
Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?
You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.
Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?
Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.