Phire 动物组织直接 PCR 试剂盒(不含采样工具)
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Phire 动物组织直接 PCR 试剂盒(不含采样工具)
Thermo Scientific™

Phire 动物组织直接 PCR 试剂盒(不含采样工具)

Thermo Scientific Phire 动物组织直接 PCR 试剂盒 可直接从多种动物(包括小鼠、鱼类、鸟类和昆虫了解更多信息
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货号数量
F140WH500 reactions
货号 F140WH
价格(CNY)
2,744.00
飞享价
Ends: 31-Dec-2025
3,919.00
共减 1,175.00 (30%)
Each
添加至购物车
数量:
500 reactions
请求批量或定制报价
价格(CNY)
2,744.00
飞享价
Ends: 31-Dec-2025
3,919.00
共减 1,175.00 (30%)
Each
添加至购物车
Thermo Scientific Phire 动物组织直接 PCR 试剂盒 可直接从多种动物(包括小鼠、鱼类、鸟类和昆虫)组织扩增 DNA。该试剂盒包含直接从动物组织扩增 DNA 所需的所有必要成分:优化的 PCR 试剂,稀释液和 DNARelease 添加剂——可改善 DNA 从动物组织的释放。此外,该试剂盒还含有阳性对照反应的对照引物,该对照引物同时适用于多种动物物种。随附一份介绍不同类型组织处理实验方案的详细手册。

产品优势

• 无需耗时且昂贵的 DNA 纯化步骤
• 所需的样品材料极少
• 针对不同应用提供了两种简单的实验方案
Phire 热启动 II DNA 聚合酶可以较短的延伸时间提供更高的特异性产物产量(20 s/kb)。

Phire DNA 聚合酶的最佳退火温度可能与 Taq 类聚合酶不同。

为获取最优结果,首先应采用我们的 TM 计算器准确计算您所需的 Tm。
仅供科研使用。不可用于诊断程序。
规格
热启动内置热启动
反应次数500 次反应
突出端平末端
聚合酶Phire 热启动 II DNA 聚合酶
产品类型Direct PCR 试剂盒
数量500 reactions
反应形式分离组分
样品类型组织
运输条件湿冰或干冰
原始材料DNA
高 GC PCR 扩增效果
PCR 方法直接 PCR
反应速度快速
Unit SizeEach
内容与储存
包含:
• 200 μL Phire 热启动 II DNA 聚合酶
• 5 x 1.25 mL 2X Phire 动物组织 PCR 缓冲液(含 dNTP 和 MgCl2)
• 40 μL 对照引物混合物(各 25 μM)
• 2 x 5 mL 稀释缓冲液
• 3 x 100 μL DNARelease 添加剂
• 3 x 1 mL 凝胶上样染料

在 -20°C 下储存。

常见问题解答 (FAQ)

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

How are samples, stored at different conditions, compatible with the Phire Tissue Direct PCR Master Mix?

With the Phire Tissue Direct PCR Master Mix you can amplify DNA from differently stored tissues (fresh, frozen, FFPE) from human and a variety of animal species.

Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?

You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?

Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.