Fluo-3，AM，细胞渗透性

引用和文献 (1252)

Invitrogen™

Fluo-3，AM，细胞渗透性

标记钙指示剂是结合 Ca2+ 离子后荧光强度增加的分子。Fluo-3 应用在流式细胞分析上，如涉及笼状螯合剂光活化、第二信使和神经递质的实验以及细胞水平的药理学筛选，用于 Ca2+ 的空间动力学成像。Fluo-4 是了解更多信息

货号	数量
F14218	1 mL

货号 F14218

价格（CNY)

7,121.00

添加至购物车

价格（CNY)

7,121.00

添加至购物车

标记钙指示剂是结合 Ca²⁺ 离子后荧光强度增加的分子。Fluo-3 应用在流式细胞分析上，如涉及笼状螯合剂光活化、第二信使和神经递质的实验以及细胞水平的药理学筛选，用于 Ca²⁺ 的空间动力学成像。Fluo-4 是 fluo-3 的类似物，其中两个氯取代基被氟取代，导致在 488 nm 波长处的荧光激发增强，因此荧光信号水平更高。将溶解后的指示剂直接加入含有培养细胞的培养皿中，即可向细胞上样 AM 酯形式的这类钙离子指示剂。这些指示剂适用于荧光和共聚焦显微镜、流式细胞分析和微孔板筛选应用。

了解更多有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的信息›

钙指示剂（AM 酯）规格：
•标签（Ca²⁺– 结合形式的激发/发射波长）：Fluo-3 (506/526 nm)
• 结合 Ca²⁺ 后荧光强度增加：>100 倍
• 在缓冲液中与 Ca²⁺ 结合的 K_d：∼335 nM
• 结合 Ca²⁺ 后，荧光增加，波长稍有变化

使用 TPEN 控制重金属阳离子
此外，基于 BAPTA 的指示剂可结合各种重金属阳离子（例如 Mn²⁺、Zn²⁺、Pb⁺²），亲和力远高于 Ca²⁺。可以使用重金属选择性螯合剂 TPEN 来控制由这些离子引起的钙测量值扰动。

荧光钙指示剂的更多选择
我们提供大量的 Molecular Probes™ 钙指示剂供各种实验场景选择使用。更多信息请参阅《Molecular Probes™ 手册》中的可见光激发的荧光 Ca²⁺ 指示剂—第 19.3 节。

对于 UV 激发的 Ca²⁺ 指示剂、基于蛋白的 Ca²⁺ 指示剂、Ca²⁺ 指示剂的偶联物以及其他金属离子（即 Mg²⁺、Zn²⁺）的荧光指示剂，请查看 Molecular Probes™ 手册中的 Ca²⁺、Mg²⁺、Zn²⁺ 以及其他金属离子指示剂—第 19 章。

仅供科研使用。不可用于人或动物的治疗或诊断。

仅供科研使用。不可用于诊断程序。

最大浓度1 mM

检测方法荧光

染料类型基于荧光染料

数量1 mL

运输条件室温

适用于（应用）细胞活力、增殖与细胞成像

适用于（设备）共聚焦显微镜, 荧光显微镜, 高内涵分析仪, HTS 读数仪, 微孔板读数仪, 荧光成像仪

产品类型染料

Unit SizeEach

内容与储存

在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The K_d value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (1252)

引用和文献

Abstract

Calreticulin couples calcium release and calcium influx in integrin-mediated calcium signaling.

Authors:Kwon MS,Park CS,Choi K,Ahnn J,Kim JI,Eom SH,Kaufman SJ,Song WK

Journal:Molecular biology of the cell

PubMed ID:10749940

The engagement of integrin Î±7 in E63 skeletal muscle cells by laminin or anti-Î±7 antibodies triggered transient elevations in the intracellular free Ca(2+) concentration that resulted from both inositol triphosphate-evoked Ca(2+) release from intracellular stores and extracellular Ca(2+) influx through voltage-gated, L-type Ca(2+) channels. The extracellular domain of integrin Î±7 ... More

Cellular alterations produced by the experimental increase in intracellular calcium and the nature of protective effects from pretreatment with nimodipine.

Authors:Danks AM,Hammond DN,Wainer BH,Van Buskirk RG,Isaacson RL

Journal:Brain research. Molecular brain research

PubMed ID:1334195

The immortalized septal cell line, SN56 B5 G4, generated by the fusion of mouse septal area cells and neuroblastoma cells, was used to determine if nimodipine, an antagonist of voltage sensitive calcium 'L' channels, might act in a neuroprotective fashion when intracellular calcium levels were raised by incubation in ouabain ... More

Morphine-3-glucuronide's neuro-excitatory effects are mediated via indirect activation of N-methyl-D-aspartic acid receptors: mechanistic studies in embryonic cultured hippocampal neurones.

Authors:Hemstapat K,Monteith GR,Smith D,Smith MT

Journal:Anesthesia and analgesia

PubMed ID:12873944

Indirect evidence indicates that morphine-3-glucuronide (M3G) may contribute significantly to the neuro-excitatory side effects (myoclonus and allodynia) of large-dose systemic morphine. To gain insight into the mechanism underlying M3G's excitatory behaviors, we used fluo-3 fluorescence digital imaging techniques to assess the acute effects of M3G (5-500 microM) on the cytosolic ... More

Developmental regulation of neuroligand-induced responses in cultured oligodendroglia.

Authors:Belachew S, Malgrange B, Rigo JM, Rogister B, Coucke P, Mazy-Servais C, Moonen G

Journal:Neuroreport

PubMed ID:9601652

Using whole-cell patch-clamp techniques, we show that oligosphere-derived oligodendrocyte progenitor cells (OP) display GABA-, glutamate-, 5-HT-, glycine- and acetylcholine-gated inward currents. When OP differentiate into oligodendrocytes (ODC), the amplitude of peak currents elicited by saturating concentrations of these transmitters decreases except for 5-HT. Intracellular Ca2+ concentration changes induced by microperfusion ... More

Direct detection of uncaged glutamate and the laser photostimulation of cultured rat cortex.

Authors:Torimitsu K, Niwa O

Journal:Neuroreport

PubMed ID:9559923

The photostimulation of nerve cells using a caged compound is very useful because it is non-invasive and non-destructive compared with standard electrophysiological techniques. There are no methods, however, for continuously measuring the photo-uncaged 'free' compound concentration at high temporal and spatial resolutions which can detect how much uncaged compound has ... More

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