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引用和文献 (99)
Invitrogen™
FluoReporter™lacZ 流式细胞分析试剂盒
FluoReporter™lacZ 流式细胞分析试剂盒
| 货号 | 数量 |
|---|---|
| F1930 又称 F-1930 | 1 Kit |
货号 F1930
又称 F-1930
价格（CNY)
6,779.00
飞享价
Ends: 31-Dec-2026
9,008.00共减 2,229.00 (25%)
Each
数量:
1 Kit
价格（CNY)
6,779.00
飞享价
Ends: 31-Dec-2026
9,008.00共减 2,229.00 (25%)
Each
The FluoReporter™ lacZ Flow Cytometry Kit provides materials and protocols for quantitating β-galactosidase activity with fluorescein di-V-galactoside (FDG) in single cells using flow cytometry. This kit is accompanied by a license to practice patented techniques for loading FDG by hypotonic shock and improving retention of fluorescein in lacZ-positive cells.
FluoReporter™ lacZ 流式细胞分析试剂盒可提供使用流式细胞分析及荧光素二-V-半乳糖苷 (FDG) 来定量测定单个细胞内 β-半乳糖苷酶活性的材料和方案。该试剂盒附带许可，可实施通过低渗休克加载 FDG 和改善 lacZ 阳性细胞中荧光素保留的专利技术。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
形式溶液
产品线FluoReporter
产品类型β-半乳糖苷酶报告基因测定系统
数量1 Kit
运输条件室温
Unit SizeEach
内容与储存
含1小瓶 FDG（0.5 mL，20 mM 水/DMSO/乙醇溶液，8:1:1，v/v）、1小瓶 PETG（2.0 mL，50 mM 水溶液）、氯喹二磷酸盐（1.0 mL，30 mM 水溶液）和1小瓶碘化丙啶（1.0 mL，150 µM 水溶液）。
在冷冻冰箱（-5 至 -30°C）中避光储存。
在冷冻冰箱（-5 至 -30°C）中避光储存。
引用和文献 (99)
引用和文献
Abstract
Detection of rare antigen-presenting cells by the lacZ T-cell activation assay suggests an expression cloning strategy for T-cell antigens.
Journal:Proc Natl Acad Sci U S A
PubMed ID:1378619
'The alpha/beta T-cell receptor a complex ligand formed by the association of antigenic peptides with molecules of the major histocompatibility complex (MHC). The inherent limitations of the conventional T-cell activation assays used to detect these peptide/MHC ligands have, until now, hampered the development of expression cloning systems for T-cell antigens.
Primitive adult hematopoietic stem cells can function as osteoblast precursors.
Journal:Proc Natl Acad Sci U S A
PubMed ID:14673088
'Osteoblasts are continually recruited from stem cell pools to maintain bone. Although their immediate precursor is a plastic-adherent mesenchymal stem cell able to generate tissues other than bone, increasing evidence suggests the existence of a more primitive cell that can differentiate to both hematopoietic and mesenchymal cells. We show here
Retroviral gene transfer is inhibited by chondroitin sulfate proteoglycans/glycosaminoglycans in malignant pleural effusions.
Journal:J Biol Chem
PubMed ID:9115227
'Gene therapy may be an important adjuvant for treating cancer in the pleural space. The initial results of retroviral gene transfer to cancer cells in malignant pleural effusions revealed that transduction was markedly inhibited, and studies to characterize the inhibitory factor(s) were performed. The inhibition was contained within the soluble,
In situ detection of transcriptionally active chromatin and genetic regulatory elements in individual viable mammalian cells.
Journal:Immunol Suppl
PubMed ID:2807403
'Using a newly developed FACS method for quantifying the expression of the Escherischia coli lacZ reporter gene in viable mammalian cells, we have obtained cloned cell lines in which the expression of lacZ is under the control of native endogenous transcription elements. We infected the murine pre-B cell 70Z/3 with
Transcriptional activity of the neuron-specific enolase (NSE) promoter in murine embryonic stem (ES) cells and preimplantation embryos.
Journal:Eur J Cell Biol
PubMed ID:7925488
'Mouse embryonic stem (ES) cells were transfected with a plasmid composed of an E. coli lacZ gene fused to 1.8 kb of rat neuron-specific enolase (NSE) promoter sequences. While this reporter construct had been shown previously to function exclusively in postmitotic neurons and neuro-endocrine cells of transgenic mice, stably transfected