Phusion™ 定点突变试剂盒（含 DH10B 感受态细胞）
Phusion™ 定点突变试剂盒（含 DH10B 感受态细胞）
Thermo Scientific™

Phusion™ 定点突变试剂盒（含 DH10B 感受态细胞）

该套装包括 Phusion 定点突变试剂盒和 DH10B 感受态细胞，这些细胞经优化可与试剂盒配合使用。Phusion 定点突变试剂盒Thermo Scientific Phusion 定点突变试剂盒是一种多功能的高效工具，可在任意类型的质粒了解更多信息
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价格（CNY)
1,981.00
Online Exclusive
Ends: 31-Dec-2025
2,829.00
共减 848.00 (30%)
Each
添加至购物车
该套装包括 Phusion 定点突变试剂盒和 DH10B 感受态细胞，这些细胞经优化可与试剂盒配合使用。

Phusion 定点突变试剂盒
Thermo Scientific Phusion 定点突变试剂盒是一种多功能的高效工具，可在任意类型的质粒 DNA 中引入点突变、插入或缺失。借助此试剂盒，使用可引入所需变化的磷酸化引物扩增整个质粒。在 5 分钟连接反应中，含有所需突变的扩增线性 PCR 产物在 T4 DNA 连接酶的作用下完成环化。然后，得到的质粒可以转化为大肠杆菌细胞。建议将 DH10B 感受态细胞与试剂盒配合使用。

Phusion DNA 聚合酶的较佳退火温度可能与基于 Taq 的聚合酶显著不同。为了获得最佳结果，请使用我们的 Tm 计算器准确计算 Tm。

•耐用且可靠的指数扩增方法
• 无需特殊载体、限制性位点或靶标质粒甲基化状态
• 无需单独步骤破坏起始模板
•Phusion 热启动 II 高保真 DNA 聚合酶可尽可能减少不需要的继发性突变
• 扩增长达 10 kb 的大质粒
• 聚合酶的热启动修饰可防止非特异性产物的扩增和 PCR 第一次循环之前不需要的引物降解
• 试剂盒内含 T4 DNA 连接酶；连接之前或之后无需纯化步骤

DH10B 感受态细胞
Thermo Scientific DH10B 感受态细胞是高效的化学感受态大肠杆菌细胞。该 DH10B 株适于克隆含甲基胞嘧啶和甲基腺嘌呤的 DNA，可高效克隆原核和真核基因组 DNA。这些细胞适用于使用质粒衍生载体构建 cDNA 文库。基因型：F– mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ (ara-leu)7697 galU galK λ– rpsL(StrR) nupG。

•高转化效率：>1 x 109 cfu/µg pUC19 DNA
• 适用于常规和高通量克隆和突变应用
• 便利，每小瓶包装可进行两次反应
• S.O.C.外生长培养基包括

相关产品
Thermo Scientific Phusion 定点突变试剂盒（不含感受态细胞）
规格
细胞类型DH10B
效率≥ 1 x 10^9
酶Maxima H Minus
适用于（应用）诱变
诱变能力单位点突变
产品线Phusion，Thermo Scientific™
产品类型定点突变试剂盒
数量20 次反应
样品类型PCR 产品
运输条件干冰
产品规格试剂盒
Unit SizeEach

常见问题解答 (FAQ)

Do Phusion DNA Polymerases add the non-template dependent 3'-A overhang?

Phusion DNA Polymerases generate blunt end products; therefore, blunt end cloning is recommended. If TA cloning is required, it can be performed by adding A overhangs to the blunt PCR product with e.g. Taq DNA Polymerase (Cat. No. EP0401). However, before adding the overhangs it is very important to remove all the Phusion DNA Polymerase by purifying the PCR product carefully, as the proofreading activity in Phusion DNA Polymerase is very strong. Any remaining Phusion DNA Polymerase will degrade the A overhangs, thus creating blunt ends again.

Can Phusion DNA Polymerases extend at 1 second/kb?

Yes it is possible, especially when amplifying smaller amplicons. Processivity of Phusion DNA Polymerases is 10 times that of Pfu. We recommend extension times of 15 s/kb for Phusion Flash PCR Master Mix. 15 s/kb is a conservative value that we can promise to work with almost any amplicon. In many cases, significantly shorter extension times (0-5 s/kb) can be used without compromising the yield. What separates Phusion Flash DNA Polymerase from other fast polymerases is that all steps in the PCR protocol can be shortened, including annealing and denaturation. This results in extremely fast protocols as compared with any other polymerase.

How can I obtain the highest possible transformation efficiency with Thermo Scientific DH5α and DH10B competent cells?

To achieve the highest possible transformation efficiency with Thermo Scientific DH5α (Cat. No. EC0112) and DH10B (Cat. No. EC0113) competent cells, please follow the transformation protocol provided with the product and pay attention to the following details:

- Make sure to use wet well-pressed ice to maximize cold surface volume in all the transformation steps.
- Perform the steps quickly and accurately.
- Use chilled microcentrifuge tubes and bury the whole tube up to the cap in the wet ice.
- Do not exceed the recommended heat shock time and transfer the tube with cells back to the wet ice immediately.