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引用和文献 (47)
Invitrogen™
FUN™ 1 细胞染色剂
FUN™ 1 是一种适用于酵母菌和真菌的独特的双色荧光活力探针。FUN™ 1 染色剂能够被动扩散至多种细胞类型中，并且利用弥散分布的绿色荧光对细胞质进行初步染色。然而，在一些常见的酵母菌及真菌种属中，活细胞对染料的后续处理导致形成明显具有紧凑结构的液泡结构，显示出显著的红色荧光，并伴随绿色细胞质荧光的减少。液泡结构的形成需要细胞质膜的完整性和细胞具有代谢能力。死细胞的荧光呈亮黄绿色，且没有明显的红色结构。了解有关离子指示剂了解更多信息
| 货号 | 数量 |
|---|---|
| F7030 | 100 μL |
货号 F7030
价格（CNY)
3,412.00
Online Exclusive
Ends: 31-Dec-2025
4,474.00共减 1,062.00 (24%)
Each
数量:
100 μL
价格（CNY)
3,412.00
Online Exclusive
Ends: 31-Dec-2025
4,474.00共减 1,062.00 (24%)
Each
FUN™ 1 是一种适用于酵母菌和真菌的独特的双色荧光活力探针。FUN™ 1 染色剂能够被动扩散至多种细胞类型中，并且利用弥散分布的绿色荧光对细胞质进行初步染色。然而，在一些常见的酵母菌及真菌种属中，活细胞对染料的后续处理导致形成明显具有紧凑结构的液泡结构，显示出显著的红色荧光，并伴随绿色细胞质荧光的减少。液泡结构的形成需要细胞质膜的完整性和细胞具有代谢能力。死细胞的荧光呈亮黄绿色，且没有明显的红色结构。
了解有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的更多信息›
了解有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的更多信息›
仅供科研使用。不可用于诊断程序。
规格
细胞类型真菌细胞、酵母菌细胞
最大浓度10 mM
检测方法荧光
染料类型其他标记或染料
产品规格管、玻片
数量100 μL
运输条件室温
适用于（设备）荧光显微镜, 流式细胞仪
产品线FUN
产品类型染色剂
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。
常见问题解答 (FAQ)
Can FUN 1 stained cells be examined by flow cytometry?
Live, metabolically active fungi transport FUN 1 into vacuoles to give a red-shifted fluorescence versus green/yellow fluorescence in the nucleus and cytoplasm of dead or metabolically-inactive cells. Is this a reliable indicator of fungal viability?
引用和文献 (47)
引用和文献
Abstract
SOD2 functions downstream of Sch9 to extend longevity in yeast.
Journal:Genetics
PubMed ID:12586694
'Signal transduction pathways inactivated during periods of starvation are implicated in the regulation of longevity in organisms ranging from yeast to mammals, but the mechanisms responsible for life-span extension are poorly understood. Chronological life-span extension in S. cerevisiae cyr1 and sch9 mutants is mediated by the stress-resistance proteins Msn2/Msn4 and
Early events in macrophage killing of Aspergillus fumigatus conidia: new flow cytometric viability assay.
Journal:Clin Diagn Lab Immunol
PubMed ID:11687470
'Detailed investigations of macrophage phagocytosis and killing of Aspergillus fumigatus conidia have been limited by technical difficulties in quantifying fungal uptake and viability. In order to study early events in cell pathogen ingestion and killing, we developed a new flow cytometry assay that utilizes the fungus-specific viability dye FUN-1. Metabolically
Human immature dendritic cells efficiently bind and take up secretory IgA without the induction of maturation.
Journal:J Immunol
PubMed ID:11751952
'Immature dendritic cells (DC) reside in peripheral tissues, where they pick up and process incoming pathogens via scavenger receptors or FcR such as FcgammaR and FcepsilonR. At mucosal surfaces, IgA is the main Ig to protect the body from incoming pathogens. In addition, DC are present in high numbers at
In vitro growth and analysis of Candida biofilms.
Journal:Nat Protoc
PubMed ID:19180075
'Evaluation of fungal biofilm formation can be performed using several techniques. In this protocol, we describe methods used to form Candida biofilms on three different medical device substrates (denture strips, catheter disks and contact lenses) to quantify them and to evaluate their architecture and drug susceptibility. Biofilm formation involves adhesion
Viruses activate a genetically conserved cell death pathway in a unicellular organism.
Journal:J Cell Biol
PubMed ID:16061692
'Given the importance of apoptosis in the pathogenesis of virus infections in mammals, we investigated the possibility that unicellular organisms also respond to viral pathogens by activating programmed cell death. The M1 and M2 killer viruses of Saccharomyces cerevisiae encode pore-forming toxins that were assumed to kill uninfected yeast cells