鸟苷 5'-三磷酸，BODIPY™ FL 2'-（或-3'）-O-（N-（2-氨乙基）尿烷），三钠盐 (BODIPY™ FL GTP)
鸟苷 5'-三磷酸，BODIPY&trade; FL 2'-（或-3'）-<i>O</i>-（<i>N</i>-（2-氨乙基）尿烷），三钠盐 (BODIPY&trade; FL GTP)
引用和文献 (8)
Invitrogen™

鸟苷 5'-三磷酸，BODIPY™ FL 2'-（或-3'）-O-（N-（2-氨乙基）尿烷），三钠盐 (BODIPY™ FL GTP)

BODIPY FL 腺苷 5'-三磷酸 (A-12410) 和 BODIPY FL 鸟苷 5'-三磷酸均由了解更多信息
Have Questions?
货号数量
G12411100 µL
货号 G12411
价格（CNY)
4,623.00
Each
添加至购物车
数量:
100 µL
价格（CNY)
4,623.00
Each
添加至购物车
BODIPY FL 腺苷 5'-三磷酸 (A-12410) 和 BODIPY FL 鸟苷 5'-三磷酸均由 BODIPY FL 荧光基团通过接头连接至核糖环的 2' 或 3' 位置组成。从这些核苷类似物的光谱性质中可以明显看出荧光基团和嘌呤碱基之间的相互作用。BODIPY FL GTP 的荧光量子产量仅为 BODIPY FL ATP 的十分之一；然而，该产量在 BODIPY FL GTP 与 GTP 结合蛋白结合后可能会提高。
仅供科研使用。不可用于诊断程序。
规格
标签或染料BODIPY™ FL
产品类型鸟苷-5'-三磷酸
数量100 µL
运输条件湿冰
最大浓度5 mM
产品线BODIPY
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。

引用和文献 (8)

引用和文献
Abstract
Capillary electrophoresis assay for G protein-coupled receptor-mediated GTPase activity.
Authors:Jameson EE, Pei J, Wade SM, Neubig RR, Milligan G, Kennedy RT
Journal:Anal Chem
PubMed ID:17263349
'We describe a capillary electrophoresis (CE) assay to detect G protein-coupled receptor (GPCR)-stimulated G protein GTPase activity in cell membranes expressing alpha2A adrenoreceptor-Galphao1 wild-type (wt) or C351I mutant fusion proteins using a fluorescent, hydrolyzable GTP analogue. As no change in total fluorescence is observed by conversion of substrate to product, ... More
Automated single-cell electroporation.
Authors:Bae C, Butler PJ
Journal:Biotechniques
PubMed ID:17068953
Identification of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay.
Authors:Surviladze Z, Waller A, Wu Y, Romero E, Edwards BS, Wandinger-Ness A, Sklar LA,
Journal:J Biomol Screen
PubMed ID:20008126
Small GTPases are key regulators of cellular activity and represent novel targets for the treatment of human diseases using small-molecule inhibitors. The authors describe a multiplex, flow cytometry bead-based assay for the identification and characterization of inhibitors or activators of small GTPases. Six different glutathione-S-transferase (GST)-tagged small GTPases were bound ... More
A direct fluorescence-based assay for RGS domain GTPase accelerating activity.
Authors:Willard FS, Kimple AJ, Johnston CA, Siderovski DP
Journal:Anal Biochem
PubMed ID:15840508
Diverse extracellular signals regulate seven transmembrane-spanning receptors to modulate cellular physiology. These receptors signal primarily through activation of heterotrimeric guanine nucleotide binding proteins (G proteins). A major determinant of heterotrimeric G protein signaling in vivo and in vitro is the intrinsic GTPase activity of the Galpha subunit. RGS (regulator of ... More
Real-time detection of basal and stimulated G protein GTPase activity using fluorescent GTP analogues.
Authors:Jameson EE, Roof RA, Whorton MR, Mosberg HI, Sunahara RK, Neubig RR, Kennedy RT
Journal:J Biol Chem
PubMed ID:15613467
Hydrolysis of fluorescent GTP analogues BODIPY FL guanosine 5 '-O-(thiotriphosphate) (BGTPgammaS) and BODIPY FL GTP (BGTP) by Galpha(i1) and Galpha was characterized using on-line capillary electrophoresis (o) laser-induced fluorescence assays in order that changes in sub-strate, substrate-enzyme complex, and product could be monitored separately. Apparent k values (V /[E]) (max ... More
View all 鸟苷 5'-三磷酸，BODIPY™ FL 2'-（或-3'）-O-（N-（2-氨乙基）尿烷），三钠盐 (BODIPY™ FL GTP) citations