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引用和文献 (28)
Invitrogen™
鸟苷 5'-O-（3-硫代三磷酸），BODIPY™ FL 硫酯钠盐（BODIPY™ FL GTP-γ-S，硫酯）
BODIPY FL GTP-γ-S 可以与 GTP 结合蛋白结合。这种荧光核苷酸 BODIPY FL 荧光基团通过了解更多信息
| 货号 | 数量 |
|---|---|
| G22183 又称 G-22183 | 50 µL |
货号 G22183
又称 G-22183
价格（CNY)
8,908.00
Each
数量:
50 µL
价格（CNY)
8,908.00
Each
BODIPY FL GTP-γ-S 可以与 GTP 结合蛋白结合。这种荧光核苷酸 BODIPY FL 荧光基团通过 GTP-γ-S 的 γ-硫醇连接形成。相对于游离染料，BODIPY FL GTP-γ-S 的荧光被淬灭 ∼90%，但与 G-蛋白结合后可以恢复。除了在结合研究方面的潜在用途之外，BODIPY FL GTP-γ-S 硫醚还是三联组氨酸核苷酸结合蛋白超家族成员 Fhit 的一种重要新底物。
仅供科研使用。不可用于诊断程序。
规格
标记方法化学标记、直接标记
标签或染料BODIPY™ FL
产品类型鸟苷 5'-O
数量50 µL
运输条件湿冰
最大浓度5 mM
产品线BODIPY
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。
引用和文献 (28)
引用和文献
Abstract
Detection of G proteins by affinity probe capillary electrophoresis using a fluorescently labeled GTP analogue.
Journal:Anal Chem
PubMed ID:14632149
'An affinity probe capillary electrophoresis (APCE) assay for guanine-nucleotide-binding proteins (G proteins) was developed using BODIPY FL GTPgammaS (BGTPgammaS), a fluorescently labeled GTP analogue, as the affinity probe. In the assay, BGTPgammaS was incubated with samples containing G proteins and the resulting mixtures of BGTPgammaS-G protein complexes and free BGTPgammaS
Designed FHIT alleles establish that Fhit-induced apoptosis in cancer cells is limited by substrate binding.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12574506
'The FHIT gene is inactivated early in the development of many human tumors, and Fhit-deficient mice have increased cancer incidence. Viral reexpression of Fhit kills Fhit-deficient cells by induction of apoptosis. Fhit, a member of branch 2 of the histidine-triad superfamily of nucleoside monophosphate hydrolases and transferases, is a diadenosine
Fhit-nucleotide specificity probed with novel fluorescent and fluorogenic substrates.
Journal:J Biol Chem
PubMed ID:10671479
'Fhit, a member of the histidine triad superfamily of nucleotide-binding proteins, binds and cleaves diadenosine polyphosphates and functions as a tumor suppressor in human epithelial cancers. Function of Fhit in tumor suppression does not require diadenosine polyphosphate cleavage but correlates with the ability to form substrate complexes. As diadenosine polyphosphates
Mechanism of regulation of prokaryotic tubulin-like GTPase FtsZ by membrane protein EzrA.
Journal:J Biol Chem
PubMed ID:17043359
'At initiation of cell division, FtsZ, a tubulin-like GTPase, assembles into a so-called Z-ring structure at the site of division. The formation of Z ring is negatively regulated by EzrA, which ensures only one ring at the midcell per cell cycle. The mechanism leading to the negative regulation of Z-ring
Use of a native affinity ligand for the detection of G proteins by capillary isoelectric focusing with laser-induced fluorescence detection.
Journal:Electrophoresis
PubMed ID:15274014
'Affinity probe capillary isoelectric focusing (CIEF) with laser-induced fluorescence was explored for detection of Ras-like G proteins. In the assay, a fluorescent BODIPY FL GTP analogue (BGTPgammaS) and G protein were incubated resulting in formation of BGTPgammaS-G protein complex. Excess BGTPgammaS was separated from BGTPgammaS-G protein complex by CIEF using