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引用和文献 (15)
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谷胱甘肽乙酯，生物素酰胺 (BioGEE)（谷胱甘肽化检测试剂）-特殊包装
生物素化谷胱甘肽乙酯 (BioGEE) 是一种细胞渗透性生物素化谷胱甘肽类似物，用于检测谷胱甘肽化反应。在氧化应激条件下，细胞可瞬时地将谷胱甘肽整合到蛋白质中。用 BioGEE 孵育应激细胞，发现这些细胞还将这种生物素化谷胱甘肽衍生物掺入到蛋白质中，便于鉴定氧化敏感的蛋白质。一旦这些细胞被固定和透化，您可以通过流式细胞仪或荧光显微镜用荧光链霉亲和素偶联物检测谷胱甘肽化反应。也可以通过质谱法或 Western了解更多信息
| 货号 | 数量 |
|---|---|
| G36000 | 10 x 100 μg |
货号 G36000
价格（CNY)
4,207.00
Each
数量:
10 x 100 μg
价格（CNY)
4,207.00
Each
生物素化谷胱甘肽乙酯 (BioGEE) 是一种细胞渗透性生物素化谷胱甘肽类似物，用于检测谷胱甘肽化反应。在氧化应激条件下，细胞可瞬时地将谷胱甘肽整合到蛋白质中。用 BioGEE 孵育应激细胞，发现这些细胞还将这种生物素化谷胱甘肽衍生物掺入到蛋白质中，便于鉴定氧化敏感的蛋白质。一旦这些细胞被固定和透化，您可以通过流式细胞仪或荧光显微镜用荧光链霉亲和素偶联物检测谷胱甘肽化反应。也可以通过质谱法或 Western 蛋白印迹法结合荧光素或酶标记的链霉亲和素偶联物，来提取和分析谷胱甘肽化蛋白质和 BioGEE。
仅供科研使用。不可用于诊断程序。
规格
数量10 x 100 μg
产品类型Glutathione Reagent
Unit SizeEach
引用和文献 (15)
引用和文献
Abstract
Glutathione directly reduces an oxidoreductase in the endoplasmic reticulum of mammalian cells.
Journal:J Biol Chem
PubMed ID:15507438
'The formation of disulfide bonds is an essential step in the folding of many glycoproteins and secretory proteins. Non-native disulfide bonds are often formed between incorrect cysteine residues, and thus the cell has dedicated a family of oxidoreductases that are thought to isomerize non-native bonds. For an oxidoreductase to be
S-glutathiolation by peroxynitrite of p21ras at cysteine-118 mediates its direct activation and downstream signaling in endothelial cells.
Journal:FASEB J
PubMed ID:16415107
'The highly reactive species, peroxynitrite, is produced in endothelial cells in pathological states in which the production of superoxide anion and NO is increased. Here, we show that peroxynitrite added exogenously or generated endogenously in response to exposure to an NO donor or oxidized low-density lipoproteins (oxLDL) increases p21ras activity
Detection and affinity purification of oxidant-sensitive proteins using biotinylated glutathione ethyl ester.
Journal:Methods Enzymol
PubMed ID:12078486
'na'
Detecting oxidative post-translational modifications in proteins.
Journal:Amino Acids
PubMed ID:17021655
Oxidative stress induces various post-translational modifications (PTM); some are reversible in vivo via enzymatic catalysis. The present paper reviews specific procedures for the detection of oxidative PTM in proteins, most of them including electrophoresis. Main topics are carbonylated and glutathionylated proteins as well as modification of selected amino acids (Cys,
Glutathione binding to the Bcl-2 homology-3 domain groove: a molecular basis for Bcl-2 antioxidant function at mitochondria.
Journal:J Biol Chem
PubMed ID:17690097
Bcl-2 protects cells against mitochondrial oxidative stress and subsequent apoptosis. However, the mechanism underlying the antioxidant function of Bcl-2 is currently unknown. Recently, Bax and several Bcl-2 homology-3 domain (BH3)-only proteins (Bid, Puma, and Noxa) have been shown to induce a pro-oxidant state at mitochondria (1-4). Given the opposing effects