透化培养基（培养基 B）

您应该加入约100 µL的细胞。为此，方法之一是分装正确数量的细胞（10E6），然后离心去上清。残留体积大约为100 µL。

可以。通常情况下，您需要延长在通透缓冲液（试剂B）中的孵育时间，以下为两份相关的文献：

•Millard I, Degrave E, Philippe M et al. (1998) Detection of intracellular antigens by flow cytometry: comparison of two chemical methods and microwave heating. Clin Chem 44:2320–2330.
•Parker JE, Mufti GJ, Rasool F et al. (2000) The role of apoptosis, proliferation, and the Bcl-2–related proteins in the myelodysplastic syndromes and acute myeloid leukemia secondary to MDS. Blood 96:3932–3938.

可以，以下为两份相关的文献：

Haranaga S, Yamaguchi H, Friedman H et al.(2001) Chlamydia pneumoniae infects and multiplies in lymphocytes In vitro. Infect Immun 69:7753–7759.
Al-Mufti R, Hambley H, Albaiges G et al. (2000) Increased fetal erythroblasts in women who subsequently develop pre-eclampsia. Hum Reprod 15:1624–1628.

会。在流式细胞术应用中，FIX & PERM细胞通透试剂盒可用于固定和通透悬浮细胞，从而使抗体可以接近到胞内靶标且不降低其散射特性。此试剂盒经验证可用于细胞系、全血和骨髓。除了固定和通透细胞外，该试剂盒还可裂解红细胞。其可作为通用标记试剂（GPR），可用于临床应用，同时也可广泛用于实验室研究。

Your cells should be in approximately 100 µL volume. One way to do this is to aliquot the correct number of cells (10E6) and then spin them down and decant the supernatant. The residual volume should be approximately 100 µL.

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