iBlot™ 3 蛋白免疫印迹转印装置

iBlot 3 蛋白免疫印迹转印装置是一款干转印装置，可在短短3分钟内高效可靠地完成蛋白免疫印迹转印，且无需液体缓冲液。iBlot 3 系统与硝酸纤维素和聚偏二氟乙烯 (PVDF) 膜兼容，与传统湿转印方法相比，可在较短的时间内提供同等或更好的性能。iBlot了解更多信息

货号	质保期
IB31001	标准，含 1 年保修期

货号 IB31001

价格（CNY)

标准，含 1 年保修期

iBlot 3 蛋白免疫印迹转印装置是一款干转印装置，可在短短3分钟内高效可靠地完成蛋白免疫印迹转印，且无需液体缓冲液。iBlot 3 系统与硝酸纤维素和聚偏二氟乙烯 (PVDF) 膜兼容，与传统湿转印方法相比，可在较短的时间内提供同等或更好的性能。iBlot 3 蛋白免疫印迹转印装置是 iBlot 干式转印系统的组成部分，该系统由转印装置和包含所需缓冲液和转印膜（硝酸纤维素或 PVDF）的转印膜组耗材组成。

iBlot 3 蛋白免疫印迹转印装置的特点包括：
• 在短短3分钟内即可完成蛋白质转印
• 高检测灵敏度，可实现均匀一致的蛋白质转印
• 两个独立控制的转印站，能够转印多达两个中型或四个小型印迹
• 内置可调冷却功能，确保一致的转印温度
• 针对低、高和广泛的分子量范围优化的预编程转印方法
• 能够创建自定义程序
• 简单、用户友好的界面

iBlot 干式转印系统的工作原理
缓冲液离子库会掺入到转印膜组的凝胶基质中，而非缓冲液槽或浸润滤纸中。凝胶基质中的高密度离子有助于实现蛋白质快速转印。在印迹过程中，铜正极不会因水电解而产生氧气，从而减少了印迹变形（传统的蛋白转印技术，包括湿转、半湿转和半干转，均使用产生氧气的惰性电极）。

使用 iBlot 干式转印系统时，由于电极之间的距离缩短、场强高且电流高，因此可缩短转印时间。在手动制备印迹夹心层的过程中常会产生夹层气泡，而此装置通过有助于均匀、完全转印的独特去气泡设计可轻松避免产生气泡。使用 iBlot 3 系统时，无需制备缓冲液或在印迹后纯化回收。每块印迹的总制备和运行时间通常不到10分钟。

仅供科研使用。不可用于诊断程序。

数量1 device

质保期标准，含 1 年保修期

适用于（应用）蛋白质免疫印迹

适用于（设备）iBlot 3 转印膜组

凝胶兼容性小型或中型：Bis-Tris 凝胶、Tricine 凝胶、Tris-Acetate 凝胶和 Tris-Glycine 凝胶

膜兼容性硝酸纤维素、PVDF

传送模式干式

产品线iBlot™ 3

运行时间约 3 至 10 分钟

通量2 块中型或4 块小型凝胶

类型转印装置

Unit SizeEach

内容与储存

• iBlot 3 凝胶转印装置
• Western Blot 滚轴

室温储存。

常见问题解答 (FAQ)

I used PVDF Gel Transfer Stacks with the iBlot 3 Western Blot Transfer System, and my proteins did not transfer to the membrane. What happened?

This is likely due to the PVDF membrane being dry or partially dry. Regions where PVDF membranes are dry appear whiter than places where the membrane is wet. Remove the membrane and reactivate in 100% methanol, and rinse in water before reapplying to the transfer stack.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

After transferring using the iBlot 3 Western Blot Transfer System, I noticed that the Bottom Stack transfer gel melted to a viscous blue solution. Why is this?

This happens if the membrane is trimmed to fit the gel size resulting in direct contact between the Top and Bottom transfer stacks. This can be avoided by maintaining the membrane size identical to the transfer stack. Transfer quality is not affected by smaller gel size compared to the membrane.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the Blotting Roller that is provided with the iBlot 3 Western Blot Transfer System?

The Blotting Roller is a plastic roller attached to a stainless steel handle. It is used to remove any air bubbles between the gel and blotting membrane during the assembly of the transfer stacks and gel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I cut the membrane in the iBlot 3 Transfer Stacks to fit my separating gel?

No, we recommend maintaining the membrane size identical to the transfer stacks. This helps ensure that there is no direct contact between the Top and Bottom transfer stacks.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Are the PVDF and nitrocellulose membranes in the iBlot 3 Transfer Stacks compatible with fluorescent antibodies or membrane staining (e.g., LI-COR, Ponceau S)?

The PVDF and nitrocellulose membranes in the iBlot 3 Transfer Stacks are compatible with all commonly used detection methods such as staining, immunodetection, fluorescence, etc.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all iBlot™ 3 Western Blot 转印装置 FAQs