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Thermo Scientific Chemicals
Pyronin Y
Marker dye for RNA useful in acid buffer systems | CAS: 92-32-0 | C17H19ClN2O | 302.802 g/mol
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化学标识符
CAS92-32-0
IUPAC Name3,6-bis(dimethylamino)-10λ⁴-xanthen-10-ylium chloride
Molecular FormulaC17H19ClN2O
InChI KeyINCIMLINXXICKS-UHFFFAOYSA-M
SMILES[Cl-].CN(C)C1=CC2=[O+]C3=CC(=CC=C3C=C2C=C1)N(C)C
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规格
规格表
规格表Appearance (Color)Green to dark green or red
lambda-max (UV-Vis)546-551nm
Specific Absorbance (E 1%/1cm)≥48000 (Lambda max)
FormPowder
Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.
Solubility
Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL).
Notes
Stable under recommended storage conditions. Incompatible with oxidizing agents.
Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.
Solubility
Soluble in water. Also soluble in methyl cellosolve (9 mg/mL) and ethanol (3 mg/mL).
Notes
Stable under recommended storage conditions. Incompatible with oxidizing agents.
RUO – Research Use Only
General References:
- Bo Li; Ying Wu; Xiao-Ming Gao. Pyronin Y as a fluorescent stain for paraffin sections. The Histochemical Journal. 2002, 34,(6-7), 299-303.
- Laura M.Andrews; Mark R.Jones; Michelle A.Digman; Enrico Gratton. Spectral phasor analysis of Pyronin Y labeled RNA microenvironments in living cells. Biomed Opt Express. 2013, 4,(1), 171-177.