4-羟乙基哌嗪乙磺酸缓冲盐,pH7.0(2X用于转染)
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4-羟乙基哌嗪乙磺酸缓冲盐,pH7.0(2X用于转染)
4-羟乙基哌嗪乙磺酸缓冲盐,pH7.0(2X用于转染)
4-羟乙基哌嗪乙磺酸缓冲盐,pH7.0(2X用于转染)
Thermo Scientific Chemicals

4-羟乙基哌嗪乙磺酸缓冲盐,pH7.0(2X用于转染)

HEPES-buffered saline (2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonic acid, CAS # 7365-45-9) is a Good’s buffer used to maintain physiological pH. | CAS: 7365-45-9 | C8H18N2O4S | 238.30 g/mol
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货号数量
J62623.AP
又称 J62623-AP
500 mL
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货号 J62623.AP
又称 J62623-AP
价格（CNY)
-
数量:
500 mL
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化学标识符
CAS7365-45-9
IUPAC Name2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
Molecular FormulaC8H18N2O4S
InChI KeyJKMHFZQWWAIEOD-UHFFFAOYSA-N
SMILESOCCN1CCN(CCS(O)(=O)=O)CC1
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规格Specification Sheet规格表
CommentAll components are dissolved in UV treated 18.2 megohm-cm ± 1 water, followed by pH adjustment and filtered through 0.22 micron filter.
Appearance (Color)Clear colorless
FormLiquid
pH7.0 ± 0.2 (1X)
HEPES is common buffer for biological sciences, particularly used in cell culture to maintain physiological pH. It acts a buffering component, which is used in the preparation of buffers. It is described as one of the best all-purpose buffers available for use in biological research.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

  • HEPES-buffered saline is an ionic buffer with a pKa=7.55
  • HEPES is an ethanesulfonic acid with a 4-(2-hydroxyethyl)piperazin-1-yl substitution within one of its methyl groups

Application

  • HEPES-buffered saline can be used to maintain physiological pH in a solution; it is frequently used for protein isolation and other molecular applications
  • Useful for cell culture, HEPES maintains pH even in the presence of carbon dioxide
  • It is intended for use in cell culture and molecular biology
RUO – Research Use Only

General References:

  1. Rochat, S.; Alberio, L. Formaldehyde-fixation of platelets for flow cytometric measurement of phosphatidylserine exposure is feasible. Cytometry A 2015, 87 (1), 32-36.
  2. Yang, H. N.; Park, J. S.; Jeon, S. Y.; Park, K. H. Carboxymethylcellulose (CMC) formed nanogels with branched poly (ethyleneimine)(bPEI) for inhibition of cytotoxicity in human MSCs as a gene delivery vehicles. Carbohydr. Polym. 2015, 122, 265-275.