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Thermo Scientific Chemicals
TRIS-buffered saline (TBS, 10X) pH 7.4, for Western blot
For Western blot washing | CAS: 77-86-1 | C4H11NO3 | 121.136 g/mol
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化学标识符
CAS77-86-1
IUPAC Name2-amino-2-(hydroxymethyl)propane-1,3-diol
Molecular FormulaC4H11NO3
InChI KeyLENZDBCJOHFCAS-UHFFFAOYSA-N
SMILESNC(CO)(CO)CO
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规格
规格表
规格表Appearance (Color)Clear colorless
FormLiquid
CommentAll components are dissolved in UV treated 18.2 megohm-cm +/- 1 water, followed by pH adjustment and 1 micron filtration
pH7.4 +/- 0.2 (1X concentration)
For Western blot washing.TRIS-buffered saline (TBS, 10X) is used as a washing buffer for electrodes, alkaline phosphatase and peroxidase conjugates in dot blot assay. Further, it acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
For Western blot washing.TRIS-buffered saline (TBS, 10X) is used as a washing buffer for electrodes, alkaline phosphatase and peroxidase conjugates in dot blot assay. Further, it acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions.
Solubility
Miscible with water.
Notes
500mM Tris, 1.5M NaCl, pH 7.4.Store in a cool place. Incompatible with strong oxidizing agents.
For Western blot washing.TRIS-buffered saline (TBS, 10X) is used as a washing buffer for electrodes, alkaline phosphatase and peroxidase conjugates in dot blot assay. Further, it acts as a pH stabilizer, which enable washing without disruption of antibody-antigen binding interactions.
Solubility
Miscible with water.
Notes
500mM Tris, 1.5M NaCl, pH 7.4.Store in a cool place. Incompatible with strong oxidizing agents.
RUO – Research Use Only
General References:
- Anastasia, A.; Barker, P. A.; Chao, M. V.; Hempstead, B. L. Detection of p75NTR Trimers: Implications for Receptor Stoichiometry and Activation. J. Neurosci. 2015, 35 (34), 11911-11920.
- Brand, C. S.; Sadana, R.; Malik, S.; Smrcka, A. V.; Dessauer, C. W. Adenylyl Cyclase 5 Regulation by G βγ Involves Isoform-Specific Use of Multiple Interaction Sites. Mol. Pharmacol. 2015, 88 (4), 758-767.