包含: • 4 x 12.5 mL Luminaris HiGreen 低浓度 ROX qPCR 预混液 (2X),含有热启动 Taq DNA 聚合酶、UDG、dNTP(包含 dUTP)和 SYBR Green I,溶于优化 PCR 缓冲液中;足以进行 5000 x 20 µL 反应。该预混液包含低浓度的 ROX Passive Reference Dye,适用于特定实时平台。 •2 x 30 mL 无核酸酶水
储存于 -20°C 下。
常见问题解答 (FAQ)
Does the yellow dye from the Yellow Sample Buffer provided with Luminaris Color and DyNAmo Flash qPCR Master Mixes influence qPCR performance?
The addition of yellow dye from the Yellow Sample Buffer to qPCR reaction has no influence on the efficiency, specificity, sensitivity or Cq values. Using the Yellow Sample Buffer is optional, but the dye helps to see in which wells the sample has been already added.
What DNA-binding dye is present in Luminaris Color HiGreen qPCR Master Mix?
Luminaris Color HiGreen master mixes contain SYBR Green I double-stranded DNA binding dye.
Does dUTP in Luminaris qPCR master mix influence qPCR efficiency?
Luminaris qPCR master mixes have all dTTP substituted with dUTP. dUTP presence does not influence the reaction speed and efficiency of the master mix. 100% substitution of dTTP with dUTP facilitates an efficient clearance of the contamination from previous PCR reactions.
What enzyme is used in Luminaris qPCR Master Mixes?
Luminaris qPCR master mixes contain Hot Start Taq DNA Polymerase. This is a Taq DNA polymerase, which has been chemically modified by the addition of heat-labile groups to amino acid residues blocking enzyme activity. The Hot Start Taq DNA Polymerase has ultra low residual activity until thermally activated. Therefore reactions can be assembled at room temperature with no risk for the extension of non-specifically annealed primers or primer dimers and providing higher specificity for DNA amplification. The enzyme is activated by 10 minutes incubation at 95 degrees C.
Can I prepare a qPCR plate in advance and store it for later use when using Luminaris qPCR Master Mixes?
When using a hot start polymerase it is not critical to start reactions immediately after setup. The Hot Start Taq DNA Polymerase in Luminaris qPCR Master Mixes has ultra low residual activity until thermally activated. Therefore reactions can be assembled and stored at room temperature for up to 72 hours with no risk for the extension of non-specifically annealed primers or primer dimers.