CCF2-AM

CCF2-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF2 substrate which allows it to readily enter cells.了解更多信息

货号	数量
K1023	5 mg

货号 K1023

价格（CNY)

283,677.00

添加至购物车

价格（CNY)

283,677.00

添加至购物车

CCF2-AM, our LiveBLAzer™ substrate, is a lipophilic, esterified form of the CCF2 substrate which allows it to readily enter cells. Upon entry, cleavage by endogenous cytoplasmic esterases rapidly converts CCF2-AM into its negatively charged form, CCF2 or CCF4, which is retained in the cytosol. CCF2 is a Fluorescence Resonance Energy Transfer (FRET) substrate which consists of a cephalosporin core linking 7-hydroxycoumarin to fluorescein. This product is to be used as a FRET-based substrate for β-lactamase as a sensitive reporter of mammalian gene expression in the GeneBLAzer® technology.

仅供科研使用。不可用于诊断程序。

细胞渗透性细胞通透性

适用于（设备）流式细胞仪、微孔板读数仪

产品线GeneBLAzer

数量5 mg

运输条件室温

底物CCF2-AM

检测方法荧光

底物属性化学底物

底物类型β-内酰胺酶底物

Unit SizeEach

内容与储存

在冰箱（-5 至 -30°C）中储存。

常见问题解答 (FAQ)

Do you offer an alternative to GeneBLAzer In Vivo Detection Kit (Cat. No. 12578134)?

The following are two alternatives to the GeneBLAzer In Vivo Detection Kit:
- LiveBLAzer FRET-B/G Loading Kit with CCF2-AM (Cat. No. K1032, K1025)
- LiveBLAzer FRET-B/G Loading Kit with CCF4-AM (Cat. No. K1095, K1096, K1030)

Concerning CCF2-AM and CCF4-AM, these molecules differ by two carbons in the bridge that links the coumarin moiety to the lactam ring. Both are in the membrane-permeable, esterified forms, and can be used for assays in intact cells. CCF4-AM has better solubility properties than CCF2-AM and is thus best suited for screening applications. In addition, CCF4-AM has slightly better FRET and thus slightly lower background than CCF2-AM.

Note: We also offer the loading solutions as a separate kit, Loading Solutions (Cat. No. K1026), and CCF2-AM is also available as a standalone product (Cat. No. K1023).

引用和文献 (1)

引用和文献

Abstract

Folate receptor alpha and caveolae are not required for Ebola virus glycoprotein-mediated viral infection.

Authors:Simmons G, Rennekamp AJ, Chai N, Vandenberghe LH, Riley JL, Bates P,

Journal:J Virol

PubMed ID:14645601

Folate receptor alpha (FRalpha) has been described as a factor involved in mediating Ebola virus entry into cells (6). Furthermore, it was suggested that interaction with FRalpha results in internalization and subsequent viral ingress into the cytoplasm via caveolae (9). Descriptions of cellular receptors for Ebola virus and its entry ... More