β-半乳糖苷酶染色试剂盒

引用和文献 (3)

Invitrogen™

β-半乳糖苷酶染色试剂盒

您可以使用 β-Gal 染色试剂盒来测定表达 lacZ 的转染细胞百分比。lacZ 细菌基因通常用作真核细胞转染实验中的报告基因构建体。lacZ 的基因产物 β-半乳糖苷酶对细胞裂解物中的蛋白水解具有抗性，并且其活性很容易测定了解更多信息

货号	数量
K146501	1 Kit

货号 K146501

价格（CNY)

6,941.00

添加至购物车

1 Kit

价格（CNY)

6,941.00

添加至购物车

您可以使用 β-Gal 染色试剂盒来测定表达 lacZ 的转染细胞百分比。lacZ 细菌基因通常用作真核细胞转染实验中的报告基因构建体。lacZ 的基因产物 β-半乳糖苷酶对细胞裂解物中的蛋白水解具有抗性，并且其活性很容易测定。β-半乳糖苷酶催化 X-gal 的水解，产生的蓝色可以在显微镜下轻松观察。

仅供科研使用。不可用于诊断程序。

检测β-Gal (lacZ) 检测

产品类型β-半乳糖苷酶染色试剂盒

数量1 Kit

底物X-Gal

靶标β-半乳糖苷酶

检测方法比色法

Unit SizeEach

内容与储存

β-半乳糖苷酶染色试剂盒包含的试剂足够对五十个 60 mm 孔板进行染色。该试剂盒包括 10X PBS（磷酸盐缓冲盐水）、X-gal、染色液 A、B 和 C、10X 固定液以及 10μg 的 pcDNA™ 3.1/His/lacZ 控制载体。将 10X PBS 储存于室温条件下。所有其他组件都应储存在 -20°C 条件下。如果储存恰当，所有试剂可以确保 6 个月稳定。

常见问题解答 (FAQ)

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

引用和文献 (3)

引用和文献

Abstract

Use of enhanced green fluorescent protein to optimize and quantitate infection of target cells with recombinant retroviruses.

Authors:Cashion LM, Bare LA, Harvey S, Trinh Q, Zhu Y, Devlin JJ

Journal:Biotechniques

PubMed ID:10337486

'Recombinant retroviral vectors are useful tools for gene transfer in both gene therapy and research applications. An enhanced form of green fluorescent protein has been incorporated into recombinant retroviruses as a marker to follow infected cells. In this paper, we extended the use of the fluorescent reporter to quantify protein ... More

Endothelial induction of fgl2 contributes to thrombosis during acute vascular xenograft rejection.

Authors:Ghanekar A, Mendicino M, Liu H, He W, Liu M, Zhong R, Phillips MJ, Levy GA, Grant DR,

Journal:J Immunol

PubMed ID:15100314

Thrombosis is a prominent feature of acute vascular rejection (AVR), the current barrier to survival of pig-to-primate xenografts. Fibrinogen-like protein 2 (fgl2/fibroleukin) is an inducible prothrombinase that plays an important role in the pathogenesis of fibrin deposition during viral hepatitis and cytokine-induced fetal loss. We hypothesized that induction of fgl2 ... More

Phosphorylation of serine 256 is required for cAMP-dependent regulatory exocytosis of the aquaporin-2 water channel.

Authors:Fushimi K, Sasaki S, Marumo F

Journal:J Biol Chem

PubMed ID:9169447

The aquaporin-2 (AQP2) vasopressin water channel is translocated to the apical membrane upon vasopressin stimulation. Phosphorylation of serine 256 of AQP2 by cAMP-dependent protein kinase has been shown, but its relation to vasopressin-regulated translocation has not been elucidated. To address this question, wild type (WT) AQP2 and a mutant with ... More