Easy-DNA™ gDNA 纯化试剂盒
Easy-DNA™ gDNA 纯化试剂盒
Invitrogen™

Easy-DNA™ gDNA 纯化试剂盒

该 Easy-DNA™ 试剂盒是一种快速、简便的方法,可从多种细胞和组织类型中分离出高质量的高分子量基因组 DNA (gDNA)。样品量可以从单个毛囊到1克哺乳动物组织不等。其他检测样品包括小鼠尾巴、植物叶、酵母菌和大肠杆菌细胞了解更多信息
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货号数量
K1800011 Kit
货号 K180001
价格(CNY)
4,497.00
Online Exclusive
Ends: 31-Dec-2026
5,622.00
共减 1,125.00 (20%)
Each
添加至购物车
数量:
1 Kit
价格(CNY)
4,497.00
Online Exclusive
Ends: 31-Dec-2026
5,622.00
共减 1,125.00 (20%)
Each
添加至购物车
该 Easy-DNA™ 试剂盒是一种快速、简便的方法,可从多种细胞和组织类型中分离出高质量的高分子量基因组 DNA (gDNA)。样品量可以从单个毛囊到1克哺乳动物组织不等。其他检测样品包括小鼠尾巴、植物叶、酵母菌和大肠杆菌细胞。这些样品产生的高质量 DNA 平均大小在 100 kb 和 200 kb 之间,适用于 PCR、DNA 杂交、基因组 DNA 文库构建和其他应用。Easy-DNA™ 试剂盒的优势包括:

可靠性 — 从细胞、组织、植物、酵母菌、大肠杆菌、血液甚至毛囊的大样品或小样品中获得高质量基因组 DNA
简单性 — 对于大多数样品类型,分离高质量 DNA 只需4个步骤,用时通常不到90分钟
多功能性—一个试剂盒得到多种细胞和组织类型优化方案的支持

简单工作流程,优质结果
多种细胞和组织类型的优化方案包含在随试剂盒提供的说明手册中。通常,小规模 gDNA 制备在微量离心管中进行,其中样品与裂解液混合,并在 65°C 下孵育10分钟。对 DNA 颗粒进行氯仿(未包含在试剂盒中)提取、沉淀和复溶后,制备完成。该试剂盒包含一种蛋白质降解试剂(与含高蛋白–的样品配合使用,如结缔组织)、用作稀释 DNA 样品共沉淀剂的贻贝糖原和 RNase 溶液。
仅供科研使用。不可用于诊断程序。
规格
洗脱体积10 to 150 μL
最终产品类型gDNA
适用于(应用)实时荧光定量 PCR (qPCR)、克隆、Southern 印迹、测序、PCR
高通量能力不兼容高通量应用(手动)
数量1 Kit
样品类型细菌, 细胞, 真菌, 植物, 组织, 组织, Virus, Whole Blood, Yeast
运输条件室温
原始材料量Bacteria: ≤109 cells
Cells: ≤108
Hair Follicles: ≤5
Mouse Tails: 1 cm
Plant: ≤50 mg
Tissue: ≤1 g
Virus: ≤750 μL
Whole Blood: ≤2 mL
Yeast: ≤10 mL
测试时间<90 分钟
产量≤850 μg
分离技术有机提取
Unit SizeEach
内容与储存
Easy-DNA™ 试剂盒包含足以用于 15–200 份样品的试剂,具体取决于样品量和类型。

该试剂盒包含:
•55 ml 溶液 A(裂解液)
• 25 ml 溶液 B(沉淀溶液)
• 100 ml TE 缓冲液
• 750 μL 贻贝糖原(2 mg/ml,溶于无菌水)
• 750 μl RNase(2 mg/ml,溶于无菌水)
• 750 μl 蛋白降解剂(5 mg/ml,溶于无菌水)

将试剂盒储存在室温下。对于长期储存,贻贝糖原、RNase 和蛋白降解剂可储存在 -20°C 下。

常见问题解答 (FAQ)

What type of samples can be used for the isolation of DNA using the Easy-DNA Kit?

DNA from the following sources has been isolated and successfully used to produce PCR products or in Southern blot experiments:

- Fresh, dried, frozen, or heparinized blood
- Tissue culture cells, both suspended and trypsinized
- Mammalian tissue
- E.coli
- Yeast cells
- Plant leaves
- Hair follicles
- Mouse tails
- Baculovirus (viral particles)

引用和文献 (20)

引用和文献
Abstract
Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection.
Authors:Zhang Z, Theurkauf WE, Weng Z, Zamore PD,
Journal:Silence
PubMed ID:23273270
'High throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq). However, non-coding RNAs such as rRNA are highly abundant and can consume >70% of sequencing reads. A common approach is to extract only polyadenylated mRNA; ... More
Participation of fad and mbt genes in synthesis of mycobactin in Mycobacterium smegmatis.
Authors:LaMarca BB, Zhu W, Arceneaux JE, Byers BR, Lundrigan MD,
Journal:J Bacteriol
PubMed ID:14702306
'Colonies of Mycobacterium smegmatis LR222 on iron-limiting (0.1 micro M Fe) minimal medium agar fluoresce under UV light due to the accumulation in the cells of the deferri form of the siderophore mycobactin. Two mutants with little or no fluorescence, designated LUN8 and LUN9, were isolated by screening colonies of ... More
Resistance to murine AIDS in offspring of mice infected with LP-BM5. Role of CD8 T cells.
Authors:Pavlovitch JH, Hulier E, Rizk-Rabin M, Marussig M, Mazier D, Joffret ML, Hoos S, Papiernik M
Journal:J Immunol
PubMed ID:8648122
'The murine-acquired immunodeficiency syndrome (MAIDS) is caused by a mixture of murine leukemia viruses (LP-BM5 MuLV). The influence of perinatal contact with retroviruses or their Ags on the response to infection was tested by infecting with LP-BM5 (MuLV) the adult offspring of mice with MAIDS. These offspring were resistant to ... More
Inducible expression of a dominant negative DNA polymerase-gamma depletes mitochondrial DNA and produces a rho0 phenotype.
Authors:Jazayeri M, Andreyev A, Will Y, Ward M, Anderson CM, Clevenger W,
Journal:J Biol Chem
PubMed ID:12645575
'We report the inducible, stable expression of a dominant negative form of mitochondria-specific DNA polymerase-gamma to eliminate mitochondrial DNA (mtDNA) from human cells in culture. HEK293 cells were transfected with a plasmid encoding inactive DNA polymerase-gamma harboring a D1135A substitution (POLGdn). The cells rapidly lost mtDNA (t1/2 = 2-3 days) ... More
Characterization and sequencing of prototypic human T-lymphotropic virus type 1 (HTLV-1) from and HTLV-1/2 seroindeterminate patient.
Authors:Waziri A, Soldan SS, Graf MD, Nagle J, Jacobson S
Journal:J Virol
PubMed ID:10666247
'Serological screening for human T-lymphotropic virus type 1 (HTLV-1) parallels the standard screening process for human immunodeficiency virus (HIV), in which samples found positive by enzyme-linked immunosorbent assay (ELISA) are confirmed with a modified Western blot procedure. There are a significant number of cases in which HTLV-1/2 ELISA-positive specimens demonstrate ... More