Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection.
Authors:Zhang Z, Theurkauf WE, Weng Z, Zamore PD,
Journal:Silence
PubMed ID:23273270
'High throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq). However, non-coding RNAs such as rRNA are highly abundant and can consume >70% of sequencing reads. A common approach is to extract only polyadenylated mRNA; ... More
Participation of fad and mbt genes in synthesis of mycobactin in Mycobacterium smegmatis.
Authors:LaMarca BB, Zhu W, Arceneaux JE, Byers BR, Lundrigan MD,
Journal:J Bacteriol
PubMed ID:14702306
'Colonies of Mycobacterium smegmatis LR222 on iron-limiting (0.1 micro M Fe) minimal medium agar fluoresce under UV light due to the accumulation in the cells of the deferri form of the siderophore mycobactin. Two mutants with little or no fluorescence, designated LUN8 and LUN9, were isolated by screening colonies of ... More
Resistance to murine AIDS in offspring of mice infected with LP-BM5. Role of CD8 T cells.
Authors:Pavlovitch JH, Hulier E, Rizk-Rabin M, Marussig M, Mazier D, Joffret ML, Hoos S, Papiernik M
Journal:J Immunol
PubMed ID:8648122
'The murine-acquired immunodeficiency syndrome (MAIDS) is caused by a mixture of murine leukemia viruses (LP-BM5 MuLV). The influence of perinatal contact with retroviruses or their Ags on the response to infection was tested by infecting with LP-BM5 (MuLV) the adult offspring of mice with MAIDS. These offspring were resistant to ... More
Inducible expression of a dominant negative DNA polymerase-gamma depletes mitochondrial DNA and produces a rho0 phenotype.
Authors:Jazayeri M, Andreyev A, Will Y, Ward M, Anderson CM, Clevenger W,
Journal:J Biol Chem
PubMed ID:12645575
'We report the inducible, stable expression of a dominant negative form of mitochondria-specific DNA polymerase-gamma to eliminate mitochondrial DNA (mtDNA) from human cells in culture. HEK293 cells were transfected with a plasmid encoding inactive DNA polymerase-gamma harboring a D1135A substitution (POLGdn). The cells rapidly lost mtDNA (t1/2 = 2-3 days) ... More
Characterization and sequencing of prototypic human T-lymphotropic virus type 1 (HTLV-1) from and HTLV-1/2 seroindeterminate patient.
Authors:Waziri A, Soldan SS, Graf MD, Nagle J, Jacobson S
Journal:J Virol
PubMed ID:10666247
'Serological screening for human T-lymphotropic virus type 1 (HTLV-1) parallels the standard screening process for human immunodeficiency virus (HIV), in which samples found positive by enzyme-linked immunosorbent assay (ELISA) are confirmed with a modified Western blot procedure. There are a significant number of cases in which HTLV-1/2 ELISA-positive specimens demonstrate ... More