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Invitrogen™

Vivid Colors™ pcDNA™6.2/C-EmGFP-GW/TOPO™ 哺乳动物表达载体

Vivid Colors™ pcDNA™6.2 荧光蛋白 TOPO™ 表达载体(图 1)使您能够快速克隆您的基因,并将其与广泛使用且充分表征的维多利亚多管发光水母荧光蛋白 (FP)了解更多信息
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货号数量
K3592020 reactions
货号 K35920
价格(CNY)
33,123.00
飞享价
Ends: 31-Dec-2026
38,738.00
共减 5,615.00 (14%)
Each
数量:
20 reactions
价格(CNY)
33,123.00
飞享价
Ends: 31-Dec-2026
38,738.00
共减 5,615.00 (14%)
Each
Vivid Colors™ pcDNA™6.2 荧光蛋白 TOPO™ 表达载体(图 1)使您能够快速克隆您的基因,并将其与广泛使用且充分表征的维多利亚多管发光水母荧光蛋白 (FP) 融合 (1, 2)。这些有效 TOPO™ 克隆载体含祖母绿荧光蛋白 (EmGFP) 或黄色荧光蛋白 (YFP),用于简单、非侵入性检测重组蛋白(图 2)。两种 FP 均已人源化,以获得优异的哺乳动物表达 (3)。Vivid Colors™ pcDNA™6.2 荧光蛋白 TOPO™ 表达载体提供:
• 用于一步法、5 分钟 TOPO™ 克隆 PCR 扩增的目的基因的拓扑异构酶 I
•用于高水平表达重组荧光融合蛋白的 CMV 启动子
• 将 EmGFP 或 YFP 与您的蛋白的 N 或 C 端融合的能力
•用于快速选择稳定细胞系的 Bsd 抗性标志物
仅供科研使用。不可用于诊断程序。
规格
构成或诱导系统组成型
输送类型转染
适用于(应用)报告基因试验,亚细胞定位
产品类型哺乳动物表达载体
数量20 reactions
报告基因GFP (EmGFP)
选择试剂(真核生物)杀稻瘟菌素
载体pcDNA
克隆方法带 Gateway™ 的 TOPO™
产品线Gateway、TOPO、Vivid Colors、pcDNA, TOPO, Vivid Colors, pcDNA
促进剂CMV
蛋白标记GFP (EmGFP)
Unit SizeEach
内容与储存
每个 pcDNA™6.2-TOPO™ 表达载体试剂盒包含两个盒。TOPO™ 盒包含线性化和拓扑异构酶 I 活化 pcDNA™6.2-TOPO™ 载体、对照模板、测序用引物、PCR 反应组分和表达对照质粒。TOPO™ 盒应在 -20°C 下储存。One Shot™ 盒含有转化试剂,包括单次使用的 50 µL One Shot™ TOP10 化学感受态大肠杆菌、S.O.C. 培养基和 pUC19 超螺旋质粒对照品等份试液。在 -80°C 下储存感受态大肠杆菌。正确存放时,所有试剂均可保证 6 个月的稳定。

常见问题解答 (FAQ)

Gateway兼容的TOPO载体同时提供一个对照模板和对照引物。可否提供对照模板的序列吗?

对照模板的序列是受专利保护的。

经β-半乳糖苷酶染色后,还能检测到细胞中的GFP荧光么?

我们推荐在进行β-半乳糖苷酶染色前观察GFP荧光。这是因为β-半乳糖苷酶染色过程会产生非常高的自发荧光,从而干扰GFP荧光的检测。 

你们推荐在荧光显微镜下使用何种滤光片组来检测EmGFP,YFP,CFP和BFP?

EmGFP,YFP,CFP和BFP均可通过标准的FITC滤光片组和参数设定来进行检测。不过,如需获得荧光信号的最佳检测结果,我们推荐用户针对每一种荧光蛋白的激发与发射波长范围来优化滤光片组。推荐的滤光片组如下所述:

EmGFP:Omega滤光片组XF100

YFP:Omega滤光片组XF1042
Chroma滤光片组41028

CFP:Omega滤光片组XF114
Chroma滤光片组31044

BFP:Omega滤光片组XF10
Chroma滤光片组31021

如需索取滤光片组的相关信息,请直接联系Omega Optical公司(www.omegafilters.com)或Chroma 技术公司(www.chroma.com)。

你们所提供的荧光蛋白(EmGFP,YFP,BFP,CFP和Cycle 3 GFP)的最强激发/发射波长分别是多少?

我们所提供荧光蛋白的激发/发射最大波长如下所述:

EmGFP:激发:487 nm;发射:509 nm
YFP:激发:514 nm;发射:527 nm
BFP:激发:308-383 nm;发射:440-447 nm
CFP:激发:452 nm;发射:505 nm
Cycle 3 GFP:第一激发波长:395 nm;第二激发波长:478 nm;发射:507 nm

你们所提供的荧光蛋白(EmGFP,YFP,CFP,BFP和Cycle 3 GFP)是否经过人源基因密码子优化?

是的,我们所提供的所有荧光蛋白(EmGFP,YFP,CFP,BFP和Cycle 3 GFP)均经过人源基因密码子优化以适合哺乳动物表达系统。 

引用和文献 (4)

引用和文献
Abstract
Ultra-high-throughput screening method for the directed evolution of glucose oxidase.
Authors:Ostafe R, Prodanovic R, Nazor J, Fischer R,
Journal:
PubMed ID:24613019
'Glucose oxidase (GOx) is used in many industrial processes that could benefit from improved versions of the enzyme. Some improvements like higher activity under physiological conditions and thermal stability could be useful for GOx applications in biosensors and biofuel cells. Directed evolution is one of the currently available methods to ... More
Development of a fluorescence-based method for monitoring glucose catabolism and its potential use in a biomass hydrolysis assay.
Authors:Haney LJ, Coors JG, Lorenz AJ, Raman DR, Anex RP, Scott MP,
Journal:Biotechnol Biofuels
PubMed ID:19019221
'The availability and low cost of lignocellulosic biomass has caused tremendous interest in the bioconversion of this feedstock into liquid fuels. One measure of the economic viability of the bioconversion process is the ease with which a particular feedstock is hydrolyzed and fermented. Because monitoring the analytes in hydrolysis and ... More
Lignin depletion enhances the digestibility of cellulose in cultured xylem cells.
Authors:Lacayo CI, Hwang MS, Ding SY, Thelen MP,
Journal:
PubMed ID:23874568
'Plant lignocellulose constitutes an abundant and sustainable source of polysaccharides that can be converted into biofuels. However, the enzymatic digestion of native plant cell walls is inefficient, presenting a considerable barrier to cost-effective biofuel production. In addition to the insolubility of cellulose and hemicellulose, the tight association of lignin with ... More
Surface carbohydrate analysis and bioethanol production of sugarcane bagasse pretreated with the white rot fungus, Ceriporiopsis subvermispora and microwave hydrothermolysis.
Authors:Sasaki C, Takada R, Watanabe T, Honda Y, Karita S, Nakamura Y, Watanabe T,
Journal:Bioresour Technol
PubMed ID:21903385
'Effects of pretreatments with a white rot fungus, Ceriporiopsis subvermispora, and microwave hydrothermolysis of bagasse on enzymatic saccharification and fermentation were evaluated. The best sugar yield, 44.9 g per 100g of bagasse was obtained by fungal treatments followed by microwave hydrothermolysis at 180°C for 20 min. Fluorescent-labeled carbohydrate-binding modules which ... More