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引用和文献 (2)
Invitrogen™
含 Sf9 细胞的 InsectSelect™ 辉光试剂盒
The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of yourgene of interest. This vector has the following了解更多信息
| 货号 | 数量 |
|---|---|
| K81001 又称 K810-01 | 1 kit |
货号 K81001
又称 K810-01
价格（CNY)
17,724.00
Each
数量:
1 kit
价格（CNY)
17,724.00
Each
The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of your
gene of interest. This vector has the following features:
• The OpIE2 promoter for constitutive expression
• The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistance
protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines
with Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.
gene of interest. This vector has the following features:
• The OpIE2 promoter for constitutive expression
• The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistance
protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines
with Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.
仅供科研使用。不可用于诊断程序。
规格
产品类型Sf9 细胞辉光试剂盒
蛋白标记位置（至您的基因）C-端
数量1 kit
载体pIZ
克隆方法限制性内切酶/MCS
产品线InsectSelect
促进剂OplE2
蛋白标记His 标签 (6x)、V5 抗原决定簇标签, V5 Epitope Tag
Unit SizeEach
内容与储存
InsectSelect™ 辉光试剂盒包括各 20 µg 超螺旋冻干 pIZT/V5-His 和 pIZT/V5-His/CAT、各 2 µg 冻干 OpIE2 正向和反向测序引物、1 g Zeocin™、Cellfectin™ 试剂、Sf9 细胞和 1 L GIBCO™ Grace 昆虫培养基。pIZT/V5-His 载体试剂盒仅包含上述表达载体和引物。将冷冻的昆虫细胞储存于液氮。将培养基和 Cellfectin™ 试剂储存于 +4°C 下。将所有其他试剂储存于 -20°C 下。正确存放时，所有试剂均可保证 6 个月的稳定。
常见问题解答 (FAQ)
培养昆虫细胞时，需要对培养箱进行加湿么？
Does the incubator need to be humidified when culturing insect cells?
引用和文献 (2)
引用和文献
Abstract
Disarming the mustard oil bomb.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12161563
Plants are attacked by a broad array of herbivores and pathogens. In response, plants deploy an arsenal of defensive traits. In Brassicaceae, the glucosinolate-myrosinase complex is a sophisticated two-component system to ward off opponents. However, this so-called
Punctin, a novel ADAMTS-like molecule, ADAMTSL-1, in extracellular matrix.
Journal:J Biol Chem
PubMed ID:11805097
Punctin (ADAMTSL-1) is a secreted molecule resembling members of the ADAMTS family of proteases. Punctin lacks the pro-metalloprotease and the disintegrin-like domain typical of this family but contains other ADAMTS domains in precise order including four thrombospondin type I repeats. Punctin is the product of a distinct gene on human