FreeStyle™ MAX 293 表达系统

FreeStyle MAX 系统是用于哺乳动物瞬时蛋白生产的完整系统。该系统结合了 Gibco FreeStyle 培养基、FreeStyle MAX 试剂和了解更多信息

货号	数量
K900010	1 个试剂盒

货号 K900010

价格（CNY)

25,023.00

添加至购物车

1 个试剂盒

价格（CNY)

25,023.00

添加至购物车

FreeStyle MAX 系统是用于哺乳动物瞬时蛋白生产的完整系统。该系统结合了 Gibco FreeStyle 培养基、FreeStyle MAX 试剂和 FreeStyle CHO-S 细胞或 FreeStyle 293 细胞。使用 FreeStyle MAX 系统时仅需要 CO₂ 孵育器和平台振荡器（含摇瓶或旋动培养）设备。

FreeStyle MAX 系统提供：
•用于瞬时转染悬浮培养物中 CHO 和 293 细胞的简单、快速方案
• 高达毫克量级的具有预期翻译后修饰的功能蛋白
• 采用 FreeStyle™ 无血清培养基简化分泌性蛋白的下游纯化
• 直接适用于扩大生产规模的方案

FreeStyle MAX 系统能够用一周内大规模功能蛋白生产替换耗时的稳定细胞系生成。

仅供科研使用。不可用于诊断程序。

细胞系FreeStyle™ 293-F 细胞

分类非动物源性、无血清

培养类型悬浮液细胞培养

输送类型转染

表达机制基于细胞的表达

表达系统哺乳动物

适用于（应用）蛋白表达

关键功能蛋白质制备

产品线Freestyle

产品类型表达系统

数量1 个试剂盒

细胞类型293 衍生细胞

转染技术脂质转染、瞬时转染

Unit SizeEach

内容与储存

将 FreeStyle 细胞储存于液氮中。将 FreeStyle MAX 试剂储存在 2–8°C 下。将 FreeStyle CHO 表达培养基、FreeStyle 293 表达培养基和 OptiPRO SFM 储存在 2–8°C 条件下的暗处。将 pCMV SPORT 储存在 -20°C 下。在推荐储存条件下，保证稳定储存 6 个月。有关其他储存信息，请参阅手册。

常见问题解答 (FAQ)

FreeStyle Max 293表达系统与FreeStyle 293表达系统之间的主要区别是什么？

除转染试剂和用于配制转染复合物的培养基不同之外，这两个系统拥有相同的组分。FreeStyle Max 293表达系统包含了FreeStyle Max试剂和OptiPRO SFM（非动物源性的无血清培养基），而FreeStyle 293表达系统则包含293fectin转染试剂和Opti-MEM I减血清培养基（无血清培养基，但不是非动物源性）。

What is the main difference between the FreeStyle Max 293 Expression System and the FreeStyle 293 Expression System?

The two systems have the same components except for the transfection reagent and the medium supplied for formation of transfection complexes. The FreeStyle Max 293 Expression System contains FreeStyle Max Reagent and OptiPRO SFM (serum-free medium that is devoid of animal-origin components), whereas the FreeStyle 293 Expression System contains 293fectin Transfection Reagent and Opti-MEM I Reduced Serum Medium (serum-free medium but not animal-origin free).

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

What is the recommended ratio of DNA and transfection reagent when transfecting Freestyle cells?

The optimal amount will vary, but we recommend starting with a 1:1 ratio of DNA and lipid. For example, 1.25 ug DNA and 1.25 ul of the reagent for one ml of suspension cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Which vectors do you recommend for use with FreeStyle system?

Generally, any mammalian expression vector should be compatible with the FreeStyle expression system. Some of the more popular choices include pcDNA3.3-TOPO TA vector (Cat. No. K8300-01), pcDNA3.4-TOPO TA vector (Cat. no. A14697), pcDNA3.2 DEST vector (Cat. No. 12489018), pCEP vector (Cat. No. V044-50), or pcDNA4/HisMax vector (Cat. No. K864-20).

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How does FreeStyle MAX reagent compare to Lipofectamine 2000 and 293fectin reagents?

Lipofectamine 2000 is designed for adherent cells. FreeStyle and 293fectin reagents are designed for suspended Hek293 and CHO cells. For transfecting 293 suspension cells, FreeStyle MAX reagent yields transfection efficiency comparable to Lipofectamine 2000 and 293fectin reagents. However, Lipofectamine 2000 and 293fectin reagents do not work well for CHO suspension cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

View all FreeStyle™ MAX 293 表达系统 FAQs