Arcturus™ Paradise™ Plus RNA 提取和分离试剂盒
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Applied Biosystems™

Arcturus™ Paradise™ Plus RNA 提取和分离试剂盒

ARCTURUS™ Paradise™ PLUS FFPE RNA 分离试剂盒通过从福尔马林固定石蜡包埋 (FFPE) 组织中提取 RNA,对数百万份先前无法获取的样品进行前所未有的基因表达分析。使用经过专门优化以克服交联了解更多信息
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货号数量
KIT0312I12 samples
货号 KIT0312I
价格(CNY)
-
数量:
12 samples
ARCTURUS™ Paradise™ PLUS FFPE RNA 分离试剂盒通过从福尔马林固定石蜡包埋 (FFPE) 组织中提取 RNA,对数百万份先前无法获取的样品进行前所未有的基因表达分析。使用经过专门优化以克服交联 FFPE 模板挑战的试剂和方法,利用低至 5 ng 的分离固定 RNA 进行扩增(图 1)。

使用 Paradise™ 质控试剂盒确保下游步骤成功进行
并非所有 FFPE 样品都含有高质量的 RNA。使用简单的实时荧光定量 PCR 研究技术确定可用于研究的理想样品,然后使用这些经预先验证的样品信心十足地开展研究工作(图 2)。

适用于 Microgenomics 的集成系统
Paradise™ PLUS 试剂系统已作为适用于 Microgenomics™ 的 ARCTURUS™ 完整系统的一部分进行验证,后者是使用少量 RNA 进行基因表达分析的集成解决方案,该试剂系统还配有 ARCTURUSXT™ 激光捕获显微切割 (LCM) 仪器,用以获取纯细胞群。适用于 Microgenomics 的 ARCTURUS™ 系统可实现准确、灵敏的微阵列测定,这些测定可揭示在整个组织样品或细胞混合物中会被掩盖的分子。

仅供科研使用。不适用于诊断用途。
仅供科研使用。不可用于诊断程序。
规格
最终产品类型总 RNA、转录组 RNA、micro RNA
适用于(应用)微阵列分析,实时荧光定量 PCR(qPCR)
适用于(设备)ArcturusXT™ LCM 仪器
高通量能力不兼容高通量应用(手动)
样品数量12 samples
产品线ARCTURUS™, Paradise™
数量12 samples
原始材料量低至单细胞。
产品规格离心柱
分离技术酶法, 离心柱
样品类型激光捕获显微切割 (LCM), 组织 (FFPE)
目标总 RNA
Unit SizeEach
内容与储存
Paradise RNA 分离试剂盒:蛋白酶 K、蛋白酶 K 缓冲液、纯化试剂、纯化柱、微量离心管。多种储存条件

常见问题解答 (FAQ)

What is the lowest amount of RNA that can be amplified using the Arcturus Paradise Plus RNA Extraction and Isolation Kit?

With this kit, you can amplify as little as 5 ng of RNA isolated from formalin-fixed, paraffin embedded (FFPE) tissues.

How do I locate the overview image on the Arcturus XT LCM Instrument?

First, optimize the visualization of the image in the live video at 2X by increasing or decreasing the brightness setting, then right-click on the overview image and select "remember settings". This resets your optimized image settings, and when you select "re-acquire overview image" you will now generate a perfectly exposed overview image.

With the ArcturusXT LCM Instrument, which laser should I use to isolate my region of interest? UV cutting laser or IR capture laser?

For a few cells: The gentle IR-only approach is the best recommendation for preserving nucleic acids and verifying that the desired material is collected on the cap. For groups of cells or large tumor regions: Mount your tissue on a membrane slide and take advantage of the power of the IR, or IR + UV cutting laser to cut around your region of interest quickly and cleanly.

What is Arcturus Laser Capture Microdissection (LCM)?

The ArcturusXT LCM Instrument has a proprietary combination of a gentle IR laser and a powerful UV laser that work in conjunction to efficiently isolate cells from frozen sections or paraffin embedded sections without changing morphology or integrity of the biological content. The IR laser helps to capture the cells of interest, and the UV laser microdissects the captured cells. This is done without affecting the morphology of the cells, and allows for visual inspection of the remaining specimen to help ensure the quality of the sample collected. In addition to the flexibility that the dual laser system provides, there is the flexibility of multiple stage inserts for various sample types. A wide-slide stage format is available for neurobiology researchers working with very large sections of brain tissue. The petri dish stage insert enables live-cell microdissection applications such as stem cell research and other rare-cell isolations.