NuPAGE™ Tris-乙酸 SDS 缓冲液试剂盒（用于 Tris-乙酸凝胶）含有1（各含）。LA0041、NP0004、NP0005、NP0007
NuPAGE&trade; Tris-乙酸 SDS 缓冲液试剂盒（用于 Tris-乙酸凝胶）含有<i>1（各含）。LA0041、NP0004、NP0005、NP0007</i>
NuPAGE&trade; Tris-乙酸 SDS 缓冲液试剂盒（用于 Tris-乙酸凝胶）含有<i>1（各含）。LA0041、NP0004、NP0005、NP0007</i>
NuPAGE&trade; Tris-乙酸 SDS 缓冲液试剂盒（用于 Tris-乙酸凝胶）含有<i>1（各含）。LA0041、NP0004、NP0005、NP0007</i>
NuPAGE&trade; Tris-乙酸 SDS 缓冲液试剂盒（用于 Tris-乙酸凝胶）含有<i>1（各含）。LA0041、NP0004、NP0005、NP0007</i>
NuPAGE&trade; Tris-乙酸 SDS 缓冲液试剂盒（用于 Tris-乙酸凝胶）含有<i>1（各含）。LA0041、NP0004、NP0005、NP0007</i>
Invitrogen™

NuPAGE™ Tris-乙酸 SDS 缓冲液试剂盒（用于 Tris-乙酸凝胶）含有1（各含）。LA0041、NP0004、NP0005、NP0007

NuPAGE Tris-乙酸 SDS 电泳缓冲液试剂盒专为在 Tris-乙酸凝胶上分离中大型分子量蛋白质而配制。此试剂盒包含如下缓冲液：• NuPAGE Tris-乙酸 SDS 电泳缓冲液了解更多信息
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货号数量
LA00501 kit
货号 LA0050
价格（CNY)
1,178.00
Each
添加至购物车
数量:
1 kit
价格（CNY)
1,178.00
Each
添加至购物车
NuPAGE Tris-乙酸 SDS 电泳缓冲液试剂盒专为在 Tris-乙酸凝胶上分离中大型分子量蛋白质而配制。此试剂盒包含如下缓冲液：
• NuPAGE Tris-乙酸 SDS 电泳缓冲液（20X，货号 LA0041）
• NuPAGE 样品还原剂（10X，250 μL，货号 NP0004）
• NuPAGE 抗氧化剂（货号 NP0005）
• NuPAGE LDS 样品缓冲液（4X，10 mL，货号 NP0007）

查看所有 SDS-PAGE 可用的缓冲液和试剂

仅供科研使用。不可用于诊断程序。
规格
凝胶类型SDS PAGE 凝胶
数量1 kit
凝胶兼容性NuPAGE™ Tris-乙酸凝胶
产品线NuPAGE
产品类型缓冲液试剂盒
Unit SizeEach
内容与储存
NuPAGE™ Tris-乙酸 SDS 缓冲液试剂盒（用于 Tris-乙酸凝胶）含有：

•20X NuPAGE™ Tris-醋酸盐 SDS 运行缓冲液 (500 mL)：在 4°C 至 25°C 下储存

• 10X NuPAGE™ 样品还原剂 (250 µL)：在 2°C 至 8°C 下储存

• NuPAGE™ 抗氧化剂 (15 mL)：在 4°C 下储存

• 4X NuPAGE™ LDS 样品缓冲液 (10 mL)：储存于 4°C 至 25°C

常见问题解答 (FAQ)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Can I use CTAB rather than SDS in my sample buffer?

No, CTAB will not work with any of our gels except for the NuPAGE Tris-Acetate gels. To use CTAB, you would need to use a running buffer of 50 mM acetic acid and 50 mM beta-alanine in equal concentrations. You would also need to switch the electrodes. Since CTAB is a cationic detergent, this would establish conditions for running a basic protein towards the anode (into the gel).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is there a reasonable replacement for the NuPAGE antioxidant for gel electrophoresis?

One can use 0.002% thioglycolic acid in the upper buffer reservoir. This is a good scavenger of free radicals. The reference to this is described by Hunkapiller et al, Methods of Enzymology, (91), 399, 1983. Caution should be taken when using this method since this compound is both toxic and expensive. In addition, the TGA must be fresh as it tends to become oxidized itself over time. Oxidized TGA will actually promote sample re-oxidation.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all NuPAGE™ Tris-乙酸 SDS 缓冲液试剂盒（用于 Tris-乙酸凝胶）含有1（各含）。LA0041、NP0004、NP0005、NP0007 FAQs