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引用和文献 (37)
Invitrogen™
MUP（4-甲基伞形酮磷酸酯，游离酸）
MUP 是碱性磷酸酶的一种荧光底物。其磷酸酯取代基由碱性磷酸酶介导的水解会产生具有蓝色荧光的 4-甲基伞形酮（激发/发射波长 ∼386/448 nm）。了解更多信息
| 货号 | 数量 |
|---|---|
| M6491 | 1 g |
货号 M6491
价格（CNY)
2,002.00
Each
数量:
1 g
价格（CNY)
2,002.00
Each
MUP 是碱性磷酸酶的一种荧光底物。其磷酸酯取代基由碱性磷酸酶介导的水解会产生具有蓝色荧光的 4-甲基伞形酮（激发/发射波长 ∼386/448 nm）。
仅供科研使用。不可用于诊断程序。
规格
激发/发射360⁄449
适用于（设备）荧光计、微孔板读数仪
标签或染料MUP（4-甲基伞形酮磷酸酯）
数量1 g
运输条件室温
底物磷酸酶底物
检测方法荧光
形式粉末
底物属性化学底物
目标酶碱性磷酸酶
Unit SizeEach
内容与储存
在冰箱（-5 至 -30°C）中储存。
引用和文献 (37)
引用和文献
Abstract
Enzyme-linked immunosorbent assay for an octapeptide based on a genetically engineered fusion protein.
Journal:Anal Chem
PubMed ID:8503503
Traditional chemical means of preparing enzyme-ligand conjugates for use in enzyme-linked immunosorbent assays (ELISAs) lead to the production of multisubstituted enzyme-ligand conjugates with a high degree of variability in the site of ligand attachment. A genetically engineered fusion protein was prepared in order to investigate the feasibility of controlled production
Rapid characterisation and identification of mycobacteria using fluorogenic enzyme tests.
Journal:Zentralbl Bakteriol
PubMed ID:8061408
'Sixty representatives of selected Mycobacterium and Nocardia species were examined for their ability to cleave 79 fluorogenic synthetic enzyme substrates based on the fluorophores 7-amino-4-methylcoumarin and 4-methylumbelliferone. The resultant data were analysed using the simple matching coefficient and clustering achieved using the unweighted pair group method with arithmetic averages algorithm.
Fluorogenic selective and differential medium for isolation of fecal streptococci.
Journal:Appl Environ Microbiol
PubMed ID:6830220
'Of 44 fluorogenic substrates tested for their ability to differentiate species of fecal streptococci, four yielded species-differentiating reactions. The remaining substrates either yielded uniformly positive, negative, or variable strain-dependent reactions. One substrate, 4-methylumbelliferone-alpha-D-galactoside, was hydrolyzed by Streptococcus bovis and S. faecium and its biotypes. 4-Methylumbelliferone-alpha-D-galactoside and a colorimetric starch substrate
Enhanced sensitivity and precision in an enzyme-linked immunosorbent assay with fluorogenic substrates compared with commonly used chromogenic substrates.
Journal:Anal Biochem
PubMed ID:16137635
'Quantitative enzyme-linked immunosorbent assay (ELISA) is a widely used tool for analyzing biopharmaceutical and vaccine products. The superior sensitivity of the ELISA format is conferred by signal amplification through the enzymatic oxidation or hydrolysis of substrates to products with enhanced color or fluorescence. The extinction coefficient for a colored product
Sensitive fluorogenic enzyme immunoassay on nitrocellulose membranes for quantitation of virus.
Journal:J Virol Methods
PubMed ID:3146582
'A highly sensitive fluorogenic enzyme-linked immunosorbent assay (FELISA), which utilizes nitrocellulose membranes as solid phase support, has been developed for the detection and identification of virus in clinical samples. Reagents were standardized and, using purified Newcastle disease virus (NDV) as a model, the theoretical lower limits of test sensitivity of