Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
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Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
Invitrogen™

Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes

The Neon NxT Electroporation System 100-μL Kit is designed specifically for use with the Neon NxT Electroporation System.
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货号反应次数
N1002525 x 2 Reactions
N1009696 x 2 Reactions
货号 N10025
价格(CNY)
9,585.00
Each
添加至购物车
反应次数:
25 x 2 Reactions
价格(CNY)
9,585.00
Each
添加至购物车

The Neon NxT Electroporation System 100-μL Kit with 1-Channel Tubes is designed specifically for use with the Neon NxT Electroporation System (Cat. Nos. NEON1S, NEON1SK, NEON18S, NEON18SK). Use this kit for transfection volumes of 100 μL, containing 5 x 105–2 x 106 adherent cells or 1 x 106–5 x 106 suspension cells. Cells that have been transfected using the included 100-μL Neon NxT tips are then ready to be washed and plated into a multi-well culture dish.

仅供科研使用。不可用于诊断程序。
规格
适用于(应用)转染
适用于(设备)Neon™ NxT Electroporation System
反应次数25 x 2 Reactions
产品类型Pipette Tip
数量25 x 2 reactions
样品容积(公制)100 μL
细胞类型Stem Cells, Primary Cells, Hard-to-Transfect Cells
产品规格Electroporation Pipette Tip
转染技术电穿孔
Unit SizeEach
内容与储存
• 10 mL Resuspension R Buffer; store at 4°C
• 10 mL Resuspension T Buffer; store at 4°C
• 10 mL Resuspension Genome Editing Buffer; store at 4°C
• 50 mL Electrolytic E100 Buffer; store at 4°C
• 25 reaction tips
• 8 electroporation 1-channel tubes

常见问题解答 (FAQ)

Should I warm Neon NxT buffers before use?

We recommend warming the buffers to room temperature before use.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I aliquot and store Neon NxT buffers?

We recommend storing Neon NxT buffers in the original containers at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What temperature should Neon NxT buffers be stored at?

Neon NxT buffers should be stored at 4 degrees C. Temperature fluctuations and storage at room temperature have been shown to increase the chances for precipitate formation. Therefore, the desired amount of buffer for an experiment should be removed from the buffer bottle and the bottle should be immediately returned to 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What state should the cells be in for best results with the Neon Nxt Electoporation System?

For best results with the Neon Nxt Electoporation System, the cells should be healthy (not stressed) and actively dividing so that the nuclear membrane integrity is already weakened for payload needing to enter the nucleus. Consider “starving” the cells of serum or other essential nutrients.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

I'm experiencing arcing issues with the Neon Nxt Electoporation System. What could be causing this?

Arcing could be caused by high voltage or pulse length settings, high salt or contaminants in the DNA sample, incorrect cell density, and air bubbles formed during mixing of cell samples. Mix samples gently to avoid air bubble formation and pipette samples in a slow, smooth motion to avoid air uptake.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.