NeuroTrace™ Nissl 500/525绿色荧光染色剂 - DMSO 溶液
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NeuroTrace™ Nissl 500/525绿色荧光染色剂 - DMSO 溶液
Invitrogen™

NeuroTrace™ Nissl 500/525绿色荧光染色剂 - DMSO 溶液

NeuroTrace Nissl 绿色荧光染色剂(仅可从 Molecular Probes 购买)可与仅存在于神经元细胞胞体中的 Nissl 物质结合了解更多信息
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货号数量
N214801 mL
货号 N21480
价格(CNY)
2,906.00
飞享价
Ends: 31-Dec-2025
3,938.00
共减 1,032.00 (26%)
Each
添加至购物车
数量:
1 mL
价格(CNY)
2,906.00
飞享价
Ends: 31-Dec-2025
3,938.00
共减 1,032.00 (26%)
Each
添加至购物车
NeuroTrace Nissl 绿色荧光染色剂(仅可从 Molecular Probes 购买)可与仅存在于神经元细胞胞体中的 Nissl 物质结合。NeuroTrace Nissl 染色剂呈现明亮的绿色荧光,可以用适合荧光素的滤光片观察,且比甲酚紫染色法的灵敏度高很多倍。NeuroTrace Nissl 绿色荧光染色剂是对经典的 FluoroNissl Green 染料的改进。
仅供科研使用。不可用于诊断程序。
规格
颜色绿色
检测方法荧光
适用于(设备)荧光显微镜
产品类型尼氏染色
数量1 mL
运输条件室温
子细胞定位尼氏体
激发/发射500/525 nm
产品线NEUROTRACE
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

常见问题解答 (FAQ)

我可以使用NeuroTrace Nissl 染色剂染色胶质或其他类型细胞,该如何改善染色效果,使其对神经元的选择性更强?

我们的NeuroTrace Nissl 染色剂可标记Nissl底物——该底物由与粗面内质网相关的核糖体RNA构成,并大量存在于神经元中。这些染料并非完全特异性靶向神经元,但由于神经元蛋白质的合成水平高而对神经元选择性染色。在某些情况下它们可能会对其他类型细胞(如神经胶质细胞)染色,所以你可能需要降低染色浓度以获得更具选择性的神经元标记效果。对于神经元染色,我们建议的稀释范围是20到300倍。

NeuroTrace Nissl染色剂可染色石蜡切片吗?

我们只在小鼠脑冰冻切片上测试过,但是有文献指出NeuroTrace Nissl 染色剂可染色石蜡切片。

NeuroTrace Nissl 染色剂是什么?

这些染料是保密的,但它们是标记Nissl物质的染色剂。Nissl物质由与粗面内质网相关的核糖体RNA构成,并大量存在于神经元细胞中。 

你们有哪些神经元示踪产品?

详细信息请查阅此网页(https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html)。

I am labeling brain cryosections with a NeuroTrace Nissl Stain. Is this compatible with antibody labeling?

Yes. We have done this successfully with an anti-GFAP primary and an Alexa Fluor secondary antibody. We would recommend labeling with the primary and secondary antibodies first, then following up with the standard NeuroTrace Nissl Stain protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (22)

引用和文献
Abstract
Beyond the olfactory bulb: an odotopic map in the forebrain.
Authors:Nikonov AA, Finger TE, Caprio J
Journal:Proc Natl Acad Sci U S A
PubMed ID:16339016
'We report electrophysiological evidence that a simple odotopy, the spatial mapping of different odorants, is maintained above the level of the olfactory bulb (OB). Three classes of biologically relevant odorants for fish are processed in distinct regions of the forebrain (FB) in the channel catfish. Feeding cues, mainly amino acids ... More
JNK-mediated induction of cyclooxygenase 2 is required for neurodegeneration in a mouse model of Parkinson's disease.
Authors:Hunot S, Vila M, Teismann P, Davis RJ, Hirsch EC, Przedborski S, Rakic P, Flavell RA
Journal:Proc Natl Acad Sci U S A
PubMed ID:14704277
'Parkinson''s disease (PD) is a neurodegenerative disorder characterized by loss of dopamine-containing neurons, but the molecular pathways underlying its pathogenesis remain uncertain. Here, we show that by eliminating c-Jun N-terminal kinases (JNKs) we can prevent neurodegeneration and improve motor function in an animal model of PD. First, we found that ... More
Defects in pancreatic development and glucose metabolism in SMN-depleted mice independent of canonical spinal muscular atrophy neuromuscular pathology.
Authors:Bowerman M, Michalski JP, Beauvais A, Murray LM, DeRepentigny Y, Kothary R,
Journal:
PubMed ID:24497575
Spinal muscular atrophy (SMA) is characterized by motor neuron loss, caused by mutations or deletions in the ubiquitously expressed survival motor neuron 1 (SMN1) gene. We recently identified a novel role for Smn protein in glucose metabolism and pancreatic development in both an intermediate SMA mouse model (Smn(2B/-)) and type ... More
Cortical control of adaptation and sensory relay mode in the thalamus.
Authors:Mease RA, Krieger P, Groh A,
Journal:
PubMed ID:24748112
A major synaptic input to the thalamus originates from neurons in cortical layer 6 (L6); however, the function of this cortico-thalamic pathway during sensory processing is not well understood. In the mouse whisker system, we found that optogenetic stimulation of L6 in vivo results in a mixture of hyperpolarization and ... More
High-throughput morphometric analysis of individual neurons.
Authors:Wu CC, Reilly JF, Young WG, Morrison JH, Bloom FE
Journal:Cereb Cortex
PubMed ID:15054070
To facilitate high-throughput quantitative analysis of neuronal structure, this study optimized the diOlistic method of whole neuron labeling to examine multiple neurons in fixed brain, and optimized image acquisition parameters to preserve signal for subsequent photoconversion. Fluorescent dye-coated gold particles were successively delivered by helium-powered ejection to 250 microm thick ... More