I am labeling brain cryosections with a NeuroTrace Nissl Stain. Is this compatible with antibody labeling?
Yes. We have done this successfully with an anti-GFAP primary and an Alexa Fluor secondary antibody. We would recommend labeling with the primary and secondary antibodies first, then following up with the standard NeuroTrace Nissl Stain protocol.
Beyond the olfactory bulb: an odotopic map in the forebrain.
Authors:Nikonov AA, Finger TE, Caprio J
Journal:Proc Natl Acad Sci U S A
PubMed ID:16339016
'We report electrophysiological evidence that a simple odotopy, the spatial mapping of different odorants, is maintained above the level of the olfactory bulb (OB). Three classes of biologically relevant odorants for fish are processed in distinct regions of the forebrain (FB) in the channel catfish. Feeding cues, mainly amino acids ... More
JNK-mediated induction of cyclooxygenase 2 is required for neurodegeneration in a mouse model of Parkinson's disease.
Authors:Hunot S, Vila M, Teismann P, Davis RJ, Hirsch EC, Przedborski S, Rakic P, Flavell RA
Journal:Proc Natl Acad Sci U S A
PubMed ID:14704277
'Parkinson''s disease (PD) is a neurodegenerative disorder characterized by loss of dopamine-containing neurons, but the molecular pathways underlying its pathogenesis remain uncertain. Here, we show that by eliminating c-Jun N-terminal kinases (JNKs) we can prevent neurodegeneration and improve motor function in an animal model of PD. First, we found that ... More
Defects in pancreatic development and glucose metabolism in SMN-depleted mice independent of canonical spinal muscular atrophy neuromuscular pathology.
Authors:Bowerman M, Michalski JP, Beauvais A, Murray LM, DeRepentigny Y, Kothary R,
Journal:
PubMed ID:24497575
Spinal muscular atrophy (SMA) is characterized by motor neuron loss, caused by mutations or deletions in the ubiquitously expressed survival motor neuron 1 (SMN1) gene. We recently identified a novel role for Smn protein in glucose metabolism and pancreatic development in both an intermediate SMA mouse model (Smn(2B/-)) and type ... More
Cortical control of adaptation and sensory relay mode in the thalamus.
Authors:Mease RA, Krieger P, Groh A,
Journal:
PubMed ID:24748112
A major synaptic input to the thalamus originates from neurons in cortical layer 6 (L6); however, the function of this cortico-thalamic pathway during sensory processing is not well understood. In the mouse whisker system, we found that optogenetic stimulation of L6 in vivo results in a mixture of hyperpolarization and ... More
High-throughput morphometric analysis of individual neurons.
Authors:Wu CC, Reilly JF, Young WG, Morrison JH, Bloom FE
Journal:Cereb Cortex
PubMed ID:15054070
To facilitate high-throughput quantitative analysis of neuronal structure, this study optimized the diOlistic method of whole neuron labeling to examine multiple neurons in fixed brain, and optimized image acquisition parameters to preserve signal for subsequent photoconversion. Fluorescent dye-coated gold particles were successively delivered by helium-powered ejection to 250 microm thick ... More