AmpErase™ 尿嘧啶 N-糖基化酶 (UNG)

AmpErase™ 尿嘧啶 N-糖基化酶 (UNG) 属于 GeneAmp™ PCR Carry-over Prevention 试剂盒组分，是一种了解更多信息

货号	数量
N8080096	100 units

货号 N8080096

价格（CNY)

1,505.00

Ends: 31-Dec-2025

2,041.00

共减 536.00 (26%)

添加至购物车

100 units

请求批量或定制报价

价格（CNY)

1,505.00

Ends: 31-Dec-2025

2,041.00

共减 536.00 (26%)

添加至购物车

AmpErase™ 尿嘧啶 N-糖基化酶 (UNG) 属于 GeneAmp™ PCR Carry-over Prevention 试剂盒组分，是一种 26 kDa 的超纯重组酶，由大肠杆菌中的尿嘧啶 N-糖基化酶基因编码，通过插入大肠杆菌宿主直接表达野生型酶。该酶可去除掺入单链或双链 DNA 中的任何尿嘧啶。

GeneAmp™ PCR Carry-over Prevention 试剂盒
GeneAmp™ PCR Carry-over Prevention 试剂盒（另售）中所提供的试剂可实现一种简单而强大的方法，确保后续 PCR 扩增中不会再次扩增 PCR 产物，从而防止出现假阳性结果。该试剂盒的特性包括：

•酶法防止 PCR 残留物污染，从而避免假阳性结果
• 可降解上游 PCR 扩增的 PCR 产物，又不会降解天然核酸模板，从而提高了扩增质量
• 不会干扰任何 PCR 或实时荧光定量 PCR 应用
• 经优化可用于 GeneAmp™ PCR 核心试剂和 GeneAmp™ 仪器系统

彻底清除 PCR 残留污染
该试剂盒使用类似于细胞的限制性修饰和切除修复系统的酶促反应，特异性降解上游 PCR 扩增中掺入 dUTP 的 PCR 产物，同时又不会降解天然核酸模板。使 PCR 产物易于降解的方法需要用 dUTP 代替 PCR 混合物中的 dTTP，并在 PCR 扩增前使用 AmpErase™ 尿嘧啶 N-糖基化酶 (UNG) 预处理所有后续 PCR 混合物。PCR 扩增产物含有尿嘧啶，可与天然含有胸苷的 DNA 模板轻松区分开来。通过 UNG 去除尿嘧啶残基，并加热降解所得的脱碱基多核苷酸，可彻底清除上游 PCR 扩增的产物。尽管 UNG 对含有 dU 的单链和双链具有活性，但 RNA 和 dUTP 自身的核糖尿嘧啶残基并非 UNG 的底物。AmpliTaq™ DNA 聚合酶、AmpliTaq Gold™ DNA 聚合酶和 PCR 混合物的其他组分在 UNG 处理过程中可保持完整活性，从而实现无 PCR 产物残留的 PCR 扩增。在印迹、克隆、测序和大多数其他 PCR 下游分析中，含 dU 的 PCR 产物的处理与含 dT 的 DNA 类似。

仅供科研使用。不可用于诊断程序。

聚合酶DNA 聚合酶

产品线AmpErase

产品类型用于 PCR 反应的尿嘧啶 N-糖基化酶

数量100 units

运输条件干冰

足够用于100 PCR 扩增

适用于（应用）常规 PCR

PCR 方法qPCR, 常规 PCR

Unit SizeEach

内容与储存

含 AmpErase™ 尿嘧啶 N-糖基化酶 (UNG)（100 单位）。足以进行 100 次 PCR 扩增，每次 100 µL。在 -20°C 下储存。

常见问题解答 (FAQ)

What is AmpErase UNG, and how does it work?

AmpErase UNG (Uracil N-glycosylase) is an enzyme utilized in a powerful method for elimination of carryover PCR products in Real-Time PCR. This method modifies PCR products such that in a new reaction, any residual products from previous PCR amplifications will be digested and prevented from amplifying, but the true DNA templates will be unaffected.

Here is how it works: During amplification, dUTP is substituted for dTTP, resulting in dUTP-containing amplicons. In subsequent reactions, a short pre-PCR incubation step will allow the AmpErase UNG to digest any dUTP containing DNA. Since AmpErase UNG is active on both single- and double-stranded dUTP-containing DNA, the procedure should work on dU-containing PCR products from standard or asymmetric PCR amplifications. However, uracil ribonucleotide residues in RNA, novel DNA containing dTTP, or cDNA containing dTTP will not be suitable substrates for UNG, so your templates will be unaffected.

Note that this is a proactive method to prevent contamination from future reactions, but will not help with a pre-existing contamination problem of standard dTTP-containing PCR products. That can only be remedied with thorough cleaning of lab surfaces, equipment and air filters.

I am using AmpErase Uracil N-glycosylase (UNG) and dUTP in my PCR reactions and would like to use the PCR product in a post-PCR application. Does the dUTP affect my ability to hybridize, sequence, clone, or digest the PCR product?

PCR products containing dUTP residues are generally equivalent to dT-containing PCR products as hybridization targets and as templates for dideoxy-terminated sequencing reactions. They can also work equivalently as targets for direct cloning as long as they are transferred into UNG-minus bacterial hosts.

The recognition of dU-containing DNA by restriction endonucleases has been studied, and depending on the specific endonuclease there may be no effect on enzymatic activity on the substitution of dU for dT (e.g., EcoR1 and BamH1), or the dU-containing DNA is cleaved more slowly than dT-containing DNA (e.g., Hpa1, HindII, and HindIII). The most accurate answer on the effects of substituting dU for dT are best determined using your own empirical studies.