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引用和文献 (6)
Invitrogen™
Oregon Green™ 488-X，琥珀酰亚胺酯，6-异构体
胺反应性 Oregon Green™ 488-X 琥珀酰亚胺酯可用于生成绿色荧光生物偶联物，最大激发/发射波长 ∼496/524 nm。荧光素的该氟化类似物克服了荧光素的一些关键局限性，包括更强的光稳定性和更低的 pKa（pKa了解更多信息
| 货号 | 数量 |
|---|---|
| O6185 又称 O-6185 | 5 mg |
货号 O6185
又称 O-6185
价格（CNY)
5,283.00
Each
数量:
5 mg
价格（CNY)
5,283.00
Each
胺反应性 Oregon Green™ 488-X 琥珀酰亚胺酯可用于生成绿色荧光生物偶联物，最大激发/发射波长 ∼496/524 nm。荧光素的该氟化类似物克服了荧光素的一些关键局限性，包括更强的光稳定性和更低的 pKa（pKa ∼4.7 vs 荧光素的 6.4），使其荧光在生理 pH 值范围内基本对 pH 值不敏感。该反应性染料在荧光基团与琥珀酰亚胺酯基团之间存在一个额外的 7 原子氨基己酰间隔 ('X')。该间隔有助于将荧光基团与连接点分离，可能减少荧光基团和其偶联的生物分子的相互作用。
仅供科研使用。不可用于诊断程序。
规格
化学反应性胺
发射524
激发496
标签或染料Oregon Green™ 488
产品类型琥珀酰亚胺酯
数量5 mg
反应一部分活性酯、琥珀酰亚胺酯
运输条件室温
标签类型经典染料
产品线Oregon Green
Unit SizeEach
内容与储存
在冷冻冰箱(-5 至 -30°C)中避光储存。
引用和文献 (6)
引用和文献
Abstract
Evolution of peptides that modulate the spectral qualities of bound, small-molecule fluorophores.
Journal:Chem Biol
PubMed ID:9862799
'BACKGROUND: Fluorophore dyes are used extensively in biomedical research to sensitively assay cellular constituents and physiology. We have created, as proof of principle, fluorophore dye binding peptides that could have applications in fluorescent dye-based approaches in vitro and in vivo. RESULTS: A panel of Texas red, Rhodamine red, Oregon green
Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. I. Chemical synthesis of various reporter group-labeled 2'-deoxyribonucleoside-5'-triphosphates.
Journal:Nucleic Acids Res
PubMed ID:12736313
Fluorescent-labeled DNA is generated through enzymatic incorporation of fluorophore-linked 2'-deoxyribonucleoside-5'-triphosphates (dNTPs) by DNA polymerases. We describe the synthesis of a variety of dye-labeled dNTPs. Amino-linker-modified 5'-triphosphates of all four naturally occurring nucleobases were used as precursors. Commercially available dyes were coupled to the amino function of the side chain. In
Cystic fibrosis transmembrane conductance regulator-independent phagosomal acidification in macrophages.
Journal:J Biol Chem
PubMed ID:17724021
It was reported recently that the cystic fibrosis transmembrane conductance regulator (CFTR) is required for acidification of phagosomes in alveolar macrophages (Di, A., Brown, M. E., Deriy, L. V., Li, C., Szeto, F. L., Chen, Y., Huang, P., Tong, J., Naren, A. P., Bindokas, V., Palfrey, H. C., and Nelson,
Noninvasive imaging of cell death using an Hsp90 ligand.
Journal:J Am Chem Soc
PubMed ID:21322555
Cell death plays a central role in normal physiology and in disease. Common to apoptotic and necrotic cell death is the eventual loss of plasma membrane integrity. We have produced a small organoarsenical compound, 4-(N-(S-glutathionylacetyl)amino)phenylarsonous acid, that rapidly accumulates in the cytosol of dying cells coincident with loss of plasma
Essential role of histidine 20 in the catalytic mechanism of Escherichia coli peptidyl-tRNA hydrolase.
Journal:Biochemistry
PubMed ID:15078105
The peptidyl-tRNA hydrolase (Pth) enzyme plays an essential role in recycling tRNA from peptidyl-tRNA that has prematurely dissociated from the ribosome. In this study of Escherichia coli Pth, the critical role of histidine 20 was investigated by site-directed mutagenesis, stopped-flow kinetic measurements, and chemical modification. The histidine residue at position