LipidSearch™ 软件

Name: LipidSearch™ 软件
SKU: OPTON-30880

使用 Thermo Scientific™ LipidSearch™ 软件从质谱数据中准确、自动鉴别及定量分析细胞脂质分子种类。

更改视图

货号	包括
OPTON-30880	从 4.0/4.1/4.2 升级到5.0
OPTON-30879	5.0 软件
B51000783	Software 5.1, upgrade from 5.0

3 选项

货号 OPTON-30880

价格（CNY)

申请报价

包括:

从 4.0/4.1/4.2 升级到5.0

脂质组学对理解细胞生理学和病理学至关重要，针对疾病表型分析的脂质谱分析是转化医学研究中发展迅速的一个领域。LC/MS 是一种用于鉴别和定量分析细胞脂质的强大技术。Thermo Scientific™ LipidSearch™ 软件可处理 LC-MS 数据，包括由 Thermo Scientific™ Orbitrap™ 质谱仪生成的高分辨率精确质量数据，从而提供准确的脂质鉴别、相对定量测定和结果可视化。该软件会自动将复杂数据汇入报告并显著缩减数据分析的时间。

Thermo Scientific™ LipidSearch™ 软件由 Ryo Taguchi 教授与 Mitsui Knowledge Industry Co.（Tokyo, Japan）联合开发，是从 LC-MS 数据进行细胞脂质自动鉴别和相对定量分析的功能强大的工具。该 LipidSearch 软件的功能如下：

与 Thermo Scientific 的三重四极杆、离子阱和 Orbitrap LC-MS 系统兼容
包含 >150 万脂质离子及其预测碎片离子的脂质数据库
从多个 LC-MS 和 LC-MSⁿ 实验获得的脂质数据对齐
LC-MS 或输注实验中鉴别的脂质前体的相对定量分析

智能峰检测可实现更加准确的鉴别和定量分析

在 LipidSearch 软件中实现的峰检测引擎可以适应多种类型质谱仪和多种类型 MS 实验的数据。为每种实验和仪器类型量身定制的独特峰检测算法组合和质谱分析处理功能，能够确保准确的峰检测，从而实现更好的鉴别和定量分析。

适用于更全面鉴别的全面脂质数据库

该数据库包含确定的结构，并包括超过150万脂质离子及其预测碎片离子。利用专业知识和实验结果计算和改进裂解谱图。还包括脂质加合物离子和 MSⁿ 指纹图谱。数据储存在 XML 文件中，而且容易进行自定义。

适应不同类型数据的双鉴别算法

LipidSearch 软件包括针对靶向鉴别进行优化的特定组 ID 算法和针对非靶向鉴别进行优化的综合 ID 算法。基团特异性算法基于极性头基团或脂肪酸，使用前体离子扫描和中性丢失扫描鉴别脂质。综合 ID 算法将产物离子扫描和数据库匹配相结合，以鉴别脂质。LipidSearch 软件还包括评分算法，以筛除掉较低概率的结果。

峰对齐可实现更好的定量分析

定量分析之前，从每份样品中提取的色谱峰在保留时间窗口内对齐，确保更准确的定量分析。

规格

适用于（设备）LC-MS 系统

包括从 4.0/4.1/4.2 升级到5.0

类型软件

Unit SizeEach

常见问题解答 (FAQ)

For TAG analysis, how efficient is the ionization and how do you ensure the accuracy of the quantification?

Triacylglycerol species form NH4 adducts under ESI conditions and species with varying numbers of double bonds typically have different response factors. One way to compensate for this is to model using lipid standards and then apply an average response factor for TG species with varying numbers of double bonds. See reference: Han, X. and R.W. Gross, Anal. Biochem. 2001, 295, 88-100.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Besides the t-test, does LipidSearch Software include ANOVA statistics or PCA?

LipidSearch Software only includes basic statistic calculations including mean, standard deviation, % RSD and p-values. To do more sophisticated statistics, use the export function to create a data table in Microsoft Excel format.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Does the ID and Quan accuracy of LipidSearch Software change with direct infusion data?

Identification in direct infusion is the same for dd-MS2 spectra. However, mixtures of different precursor ions or isomers co-isolated at the same m/z gives rise to mixture MS2 spectra. As a result, ID may be less accurate and the number of species identified is typically lower. We don't recommend LipidSearch Software for the infusion analysis of complex lipid mixtures.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can LipidSearch Software be used for Q Exactive mass spectrometry with ambient ionization sampling?

As long as the experiment is full scan and dd-MS2, the spectra can either be summed together (infusion) or treated as a chromatogram (LC-MS), and the data can be processed by LipidSearch Software.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

Can I use LipidSearch Software and TraceFinder Software without HPLC introduction?

TraceFinder Software requires chromatographic peaks for integration, but LipidSearch Software does not. LipidSearch Software can provide some limited relative quantitation from infusion data. TraceFinder Software is required for targeted LC-MS/MS methods.

Find additional tips, troubleshooting help, and resources within our Mass Spectrometry Support Center.

View all LipidSearch™ 软件 FAQs