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引用和文献 (1)
Fluorescein-Ubiquitin
Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due to了解更多信息
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| 货号 | 数量 |
|---|---|
| PV4377 | 50 μg |
| PV4378 | 500 μg |
货号 PV4377
价格（CNY)
4,646.00
Each
数量:
50 μg
价格（CNY)
4,646.00
Each
Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due to the method in labeling the ubiquitin protein, all of the lysines with the protein are available for further conjugation to form poly-ubiquitin chains.
For use in combination with FRET donors including LanthaScreen™ Tb-Ubiquitin (PV4375) or a LanthaScreen™ antibody including Tb-anti-GST (PV4216) or Tb-anti-ubiquitin-FK2 (PV4751).
For use in combination with FRET donors including LanthaScreen™ Tb-Ubiquitin (PV4375) or a LanthaScreen™ antibody including Tb-anti-GST (PV4216) or Tb-anti-ubiquitin-FK2 (PV4751).
For Research Use Only. Not for use in diagnostic procedures.
规格
检测方法Fluorescence
激发/发射340⁄490, 520
适用于（设备）Microplate Reader
标签类型Classic Dyes
标签或染料FITC (Fluorescein)
数量50 μg
读值End Point
运输条件Dry Ice
底物属性Protein-Based Substrate
技术TR-FRET
偶联物Fluorescein
形式Liquid
Unit SizeEach
内容与储存
Fluorescein-labeled ubiquitin in HEPES buffered saline. Store at -20°C.
引用和文献 (1)
引用和文献
Abstract
TR-FRET-based high-throughput screening assay for identification of UBC13 inhibitors.
Journal:J Biomol Screen
PubMed ID:22034497
UBC13 is a noncanonical ubiquitin conjugating enzyme (E2) that has been implicated in a variety of cellular signaling processes due to its ability to catalyze formation of lysine 63-linked polyubiquitin chains on various substrates. In particular, UBC13 is required for signaling by a variety of receptors important in immune regulation,