Biotin-Ubiquitin
Biotin-Ubiquitin
Invitrogen™

Biotin-Ubiquitin

Biotin-labeled ubiquitin for use as a general research tool, or in high throughput assays to monitor protein ubiquitination. Due to了解更多信息
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货号数量
PV4380100 μg
PV437910 μg
货号 PV4380
价格(CNY)
19,882.00
Each
添加至购物车
数量:
100 μg
价格(CNY)
19,882.00
Each
添加至购物车

Biotin-labeled ubiquitin for use as a general research tool, or in high throughput assays to monitor protein ubiquitination. Due to the method in labeling the ubiquitin protein, all of the lysines with the protein are available for further conjugation to form poly-ubiquitin chains.

For use in combination with other LanthaScreen™ products including LanthaScreen™ Tb-Ubiquitin (Cat. No. PV4375), Fluorescein-Ubiquitin (PV4377), or a LanthaScreen™ antibody such as Tb-anti-biotin (PV4749).

For Research Use Only. Not for use in diagnostic procedures.
规格
检测方法Fluorescence
激发/发射340/490, 520 nm
适用于(设备)Microplate Reader
标签类型Biotin & Other Haptens
标签或染料Biotin
纯度或质量等级≥90% by SDS-PAGE
数量100 μg
读值End Point
运输条件Dry Ice
底物属性Protein-Based Substrate
技术TR-FRET
偶联物Biotin
形式Liquid
Unit SizeEach
内容与储存
Biotin labeled ubiquitin in HEPES buffered saline. Store at -20°C.

引用和文献 (1)

引用和文献
Abstract
TR-FRET-based high-throughput screening assay for identification of UBC13 inhibitors.
Authors:Madiraju C, Welsh K, Cuddy MP, Godoi PH, Pass I, Ngo T, Vasile S, Sergienko EA, Diaz P, Matsuzawa S, Reed JC,
Journal:J Biomol Screen
PubMed ID:22034497
UBC13 is a noncanonical ubiquitin conjugating enzyme (E2) that has been implicated in a variety of cellular signaling processes due to its ability to catalyze formation of lysine 63-linked polyubiquitin chains on various substrates. In particular, UBC13 is required for signaling by a variety of receptors important in immune regulation, ... More