引用和文献 (1)

Predictor™ hERG 荧光偏振分析试剂盒

货号	数量
PV5365	1 Kit

货号 PV5365

价格（CNY)

19,470.00

1 Kit

价格（CNY)

19,470.00

The Predictor™ hERG Fluorescence Polarization Assay Kit provides a set of validated components to perform hERG channel biochemical binding studies in the absence of radioligand. The assay was designed to identify potential hERG channel blockers by producing data that accurately correlates with patch-clamp electrophysiology studies. The assay is based on the principle of fluorescence polarization where a red-shifted fluorescent tracer is displaced from the hERG channel by compounds that bind to the channel. Assay performance was validated with a panel of established hERG channel blockers. Results from the Predictor™ assay demonstrate a high correlation with those obtained from patch clamp techniques.

Predictor™ hERG 荧光偏振分析试剂盒提供一组经验证的组分，用于在无放射性配体的条件下进行 hERG 通道生化结合研究。此分析旨在通过生成与膜片钳电生理学研究精确相关的数据来识别潜在的 hERG 通道阻滞剂。此分析基于荧光偏振原理，其中红移荧光示踪剂在 hERG 通道被与此通道结合的化合物置换。其分析性能已使用一组广泛应用的 hERG 通道阻滞剂进行验证。由 Predictor™ 分析得出的结果与膜片钳技术得出的结果有很高的相关性。

仅供科研使用。不可用于诊断程序。

检测条目生化结合

检测方法荧光

成药靶标离子通道

适用于（设备）微孔板读数仪

基因符号hERG

包装384 孔板

产品类型检测试剂盒

蛋白质家族离子通道

数量1 Kit

读值终点法

运输条件干冰

系统类型Predictor™ 系统

目标条目hERG

偶联物Predictor hERG 红色示踪剂

产品线Predictor

Unit SizeEach

内容与储存

PV5365 Predictor™ hERG 荧光偏振分析试剂盒
（其他材料应要求单独提供；请参阅方案）
包括：
•Predictor™ hERG 膜
• Predictor™ hERG 红色示踪剂
• Predictor™ hERG 缓冲液
• E-4031
• Predictor™ hERG 荧光偏振分析方案
• 分析证书
将 Predictor™ hERG 膜储存于 -80°C。应将 Predictor™ 示踪剂红色、Predictor™ 缓冲液和 E-4301 存储在 -20°C 下。

常见问题解答 (FAQ)

I am doing drug discovery. Can I use the Predictor hERG Membranes for radioligand binding?

No, the Predictor hERG Membranes are not suitable for radioligand binding.
These membranes contain extremely high levels of hERG channel protein. Radioligand binding assays on these membranes would require either abnormally high concentrations of radioligand or severe dilutions of the membrane preparation, which tend to have a deleterious effect on hERG channel conformation and stability.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the common issues encountered with FP assays?

-No assay window: Check that your instrument is designed to do FP experiments.
-Poor discrimination between the minimum and maximum mP Control (both include the receptor): This may be an indication of the quality of the receptor. Do not make single use aliquots of the receptor or store diluted.
-The type of microplate is critical: Please see if the protocol specifies untreated polystyrene or NBS-coated plates. If the plate binds the fluorescent substrate, it cannot freely rotate and the mP reading will always be high. White plates cannot be used for FP assays. Avoid the use of silanized pipette tips and vials.
-The “No Receptor Control' (free Fluormone/Tracer) should have much higher RFU values compared to buffer alone. If not, there is a problem with the instrument setup or less commonly with the Fluormone/Tracer.
-If the free Fluormone/Tracer mP value is greater than 100 mP or negative, please refer to the user manual for your instrument and reset the G factor.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.

What is the best type of microplate reader for fluorescence polarization (FP) assays?

For FP assays, plate readers must specifically have FP built in. Please see our instrument compatibility portal (http://www.thermofisher.com/us/en/home/industrial/pharma-biopharma/drug-discovery-development/dd-misc/instrument-compatibility-portal.html).

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.

How can I test the fluorescence polarization (FP) settings of my microplate reader before doing the assay?

We offer a FP One-Step Reference Kit (https://www.thermofisher.com/order/catalog/product/P3088) to detect in green (Ex/Em = 488/535 nm) and red (Ex/Em = 525/590 nm) emission ranges, suitable for the PolarScreen Green and Red Assays. The alternative is to perform the Controls in the respective assay kit prior to performing the actual assay using test compounds.

Find additional tips, troubleshooting help, and resources within our Drug Discovery & Development Support Center.

引用和文献 (1)

引用和文献

Abstract

Development of the predictor HERG fluorescence polarization assay using a membrane protein enrichment approach.

Authors:Piper DR, Duff SR, Eliason HC, Frazee WJ, Frey EA, Fuerstenau-Sharp M, Jachec C, Marks BD, Pollok BA, Shekhani MS, Thompson DV, Whitney P, Vogel KW, Hess SD,

Journal:Assay Drug Dev Technol

PubMed ID:18471075

The life-threatening consequences of acquired, or drug-induced, long QT syndrome due to block of the human ether-a-go-go-related gene (hERG) channel are well appreciated and have been the cause of several drugs being removed from the market in recent years because of patient death. In the last decade, the propensity for ... More