Invitrogen™

BacMam 增强剂溶液 (1000X)

The BacMam Enhancer helps to increase expression of BacMam reagents in mammalian cells.To use, simply add the BacMam enhancer to了解更多信息

货号	数量
PV5835	150 μL

货号 PV5835

价格（CNY)

1,328.00

150 μL

价格（CNY)

1,328.00

The BacMam Enhancer helps to increase expression of BacMam reagents in mammalian cells.

To use, simply add the BacMam enhancer to cell culture media with or without serum that have already been treated with the BacMam reagent, then incubate cells and replace with fresh medium. Different cell types vary in their response to the BacMam enhancer and for best results the amount and treatment time will need to be optimized.

仅供科研使用。不可用于诊断程序。

细胞类型哺乳动物细胞

最大浓度1000 X

描述组蛋白脱乙酰酶抑制剂

适用于（应用）基于 BacMam 的细胞检测

数量150 μL

运输条件干冰

产品类型BacMam 增强剂溶液

Unit SizeEach

内容与储存

浓度：1.5 mM (1000X)
储存在 -20°C 下。

常见问题解答 (FAQ)

我使用你们的CellLight BacMam 试剂对我的细胞器进行荧光蛋白标记，但是标记效果不佳。我该如何加以改善？

首先，确定你们的细胞是哺乳动物且非血源性细胞。如果细胞转导效率不佳（例如只有少数细胞被标记或是标记荧光很暗），先试着增加每个细胞的微粒数。接下来试着使用BacMam Enhancer Solution。但是，如果你们的细胞显示标记而标记定位似乎太过普遍或是无处不在，那么你们应该降低每个细胞的微粒数。该手册给出了计算每个细胞的微粒数的指南和公式。

I am using your CellLight BacMam reagents for fluorescent protein-labeling of my cell organelles, but they aren't labeling well. How can I improve it?

First, make sure that your cells are mammalian and non-blood derived. If the cells have low transduction efficiency (i.e., only a few cells are labeling or are labeling only dimly), first try increasing the particle number per cell. The next thing to try is to use the BacMam Enhancer Solution. If, however, your cells show labeling, but the localization seems to be too generalized or ubiquitous within the cell, then you should reduce the particle number per cell. The manual gives a guideline and formula for calculating particle number per cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.