Nanoparticles as fluorescence labels: is size all that matters?
Authors:Swift JL, Cramb DT,
Journal:Biophys J
PubMed ID:18390610
'Fluorescent labels are often used in bioassays as a means to detect and characterize ligand-receptor binding. This is due in part to the inherently high sensitivity of fluorescence-based technology and the relative accessibility of the technique. There is often little concern raised as to whether or not the fluorescent label ... More
Measuring an antibody affinity distribution molecule by molecule.
Authors:Temirov JP, Bradbury AR, Werner JH,
Journal:Anal Chem
PubMed ID:18847284
Single molecule fluorescence microscopy was used to observe the binding and unbinding of hapten decorated quantum dots to individual surface immobilized antibodies. The fluorescence time history from an individual antibody site can be used to calculate its binding affinity. While quantum dot blinking occurs during these measurements, we describe a ... More
A two-photon excitation fluorescence cross-correlation assay for a model ligand-receptor binding system using quantum dots.
Authors:Swift JL, Heuff R, Cramb DT
Journal:Biophys J
PubMed ID:16299079
Two-photon excitation fluorescence cross-correlation spectroscopy (TPE-XCS) is a very suitable method for studying interactions of two distinctly labeled fluorescent molecules. As such, it lends itself nicely to the study of ligand-receptor interactions. By labeling the ligand with one color of fluorescent dye and the receptor with another, it is possible ... More