Quant-iT™ RNA 定量试剂盒和 Quant-iT RNA HS 试剂
Use 96- and 384-well Microplates for Fluorescence-based Assays with Quant-iT assays for optimal results
Quant-iT™ RNA 定量试剂盒和 Quant-iT RNA HS 试剂
Actual product may vary
Quant-iT™ RNA 定量试剂盒和 Quant-iT RNA HS 试剂
Quant-iT™ RNA 定量试剂盒和 Quant-iT RNA HS 试剂
Invitrogen™

Quant-iT™ RNA 定量试剂盒和 Quant-iT RNA HS 试剂

使用荧光 Quant-iT RNA 试剂和测定试剂盒可实现对高丰度 RNA 样品的高选择性和准确定量。相对于双链 DNA,这些试剂盒对 RNA 具有高度选择性,其提供的材料足够以96孔微孔板规格进行至少2000次测定。
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货号数量检测定量范围
Q331401 kitRNA 定量5 至 100 ng
Q102131,000 assaysRNA 定量,宽范围20 至 1000 ng
Q332251000 assaysRNA 定量,扩展范围200 至 10000 ng
Q328841 mLRNA 定量,高灵敏度5 至 100 ng
货号 Q33140
价格(CNY)
4,841.00
飞享价
Ends: 31-Dec-2025
6,118.00
共减 1,277.00 (21%)
Each
添加至购物车
数量:
1 kit
检测:
RNA 定量
定量范围:
5 至 100 ng
价格(CNY)
4,841.00
飞享价
Ends: 31-Dec-2025
6,118.00
共减 1,277.00 (21%)
Each
添加至购物车
使用 Quant-iT RNA 定量试剂盒可以实现简单、准确的 RNA 定量,该试剂盒具有宽范围 (BR) 和扩展范围 (XR) 配置且含有独立的高灵敏度 (HS) 试剂。相较于 dsDNA,这些 Quant-iT RNA 定量试剂盒对 RNA 具有高度选择性,可用于实现基于荧光的线性 RNA 检测范围。
Quant-iT RNA 试剂和测定试剂盒
Quant-iT RNA 试剂和测定试剂盒使 RNA 定量分析变得轻松、准确。该试剂盒提供浓缩的检测试剂、稀释缓冲液以及预稀释的 RNA 标准品。相较于双链 DNA,此测定试剂盒对 RNA 具有高度选择性,并提供 5-100 ng RNA 的动态范围。

Quant-iT RNA BR(宽范围)测定试剂盒
Quant-iT RNA BR(宽范围)测定试剂盒提供了一种准确且具有选择性的高丰度 RNA 样品定量方法。原装 Quant-iT RNA 试剂盒具有很高的灵敏度,而 Quant-iT RNA 宽范围试剂盒适用于 RNA 浓度较高的微阵列样品。相较于双链 DNA,此测定试剂盒对 RNA 具有高度选择性,并提供 20-1000 ng RNA 的动态范围。

Quant-iT RNA XR(扩展范围)测定试剂盒
Quant-iT RNA XR(扩展范围)测定试剂盒提供了一种准确且具有选择性的高丰度 RNA 样品定量方法。该检测试剂对于 RNA 具有高度选择性,不能定量 DNA、蛋白或游离核苷酸。该测定试剂盒设计用于准确检测初始浓度为 10 ng/µL 至 10,000 ng/µL 的 RNA 样品(取决于样品体积),线性检测范围为 200–10,000 ng。

每次测定,只需使用提供的缓冲液对试剂进行稀释,加入样品(1 µL 至 20 µL 的任意体积均可接受),然后就可使用基于荧光的酶标仪读取浓度值。对常见污染物,如盐、游离核苷酸、溶剂、洗涤剂和蛋白质等,有着很好的耐受性。

仅供科研使用。不可用于诊断程序。
规格
检测RNA 定量
适用于(设备)微孔板读数仪
反应次数1000次反应(200 μL 测定体积)
产品线Quant-iT
定量范围5 至 100 ng
数量1 kit
运输条件室温
检测方法荧光
Unit SizeEach

常见问题解答 (FAQ)

为什么我用Qubit Assay检测时会得到负的荧光值?

负的荧光值物理上是不可能的。它是由于软件自动扣除背景信号而造成的假象。这意味着你的荧光计检测到背景信号并将其扣除从而牺牲了真实数据。务必做一个仅有缓冲液的对照并评估信号的类型。你可能需要换用另外一块板。

我有一个Quant-iT RNA检测试剂盒,而想将它用于Qubit荧光计。可以吗?

可以,使用手册中有关于此应用的说明。

这些Quant-iT RNA试剂盒适用于siRNA和microRNA吗?

不适用,它们不能精确定量那么小的片段。Quant-iT microRNA检测试剂盒和Qubit microRNA检测试剂盒非常适合此类应用。

Quant-iT RNA检测试剂可以用于定量地高辛标记的RNA吗?

可以的,该标记物不会干扰信号。

Quant-iT RNA试剂盒的线性范围是多少?

线性范围如下:

•Quant-iT RiboGreen RNA检测试剂:1 ng/mL至1 µg/mL
•Quant-iT RNA BR检测试剂:20–1,000 ng
•Quant-iT RNA HS检测试剂:5–100 ng

引用和文献 (39)

引用和文献
Abstract
Development and validation of endogenous reference genes for expression profiling of medaka (Oryzias latipes) exposed to endocrine disrupting chemicals by quantitative real-time RT-PCR.
Authors:Zhang Z, Hu J,
Journal:Toxicol Sci
PubMed ID:17093204
The quantitative real-time reverse transcription polymerase chain reaction (Q-RT-PCR) technique has been increasingly used in endocrine disrupting chemicals (EDCs) research. Usually, an appropriate endogenous control gene is critical for Q-RT-PCR to normalize the errors and sample-to-sample variations that occur in the course of tissue collection, RNA isolation, and RT-PCR. In ... More
Corn oil supplementation to steers grazing endophyte-free tall fescue. II. Effects on longissimus muscle and subcutaneous adipose fatty acid composition and stearoyl-CoA desaturase activity and expression.
Authors:Pavan E, Duckett SK,
Journal:J Anim Sci
PubMed ID:17431049
'Eighteen steers were used to evaluate the effect of supplemental corn oil level to steers grazing endophyte-free tall fescue on fatty acid composition of LM, stearoyl CoA desaturase (SCD) activity and expression as well as cellularity in s.c. adipose. Corn oil was supplemented (g/kg of BW) at 0 (none), 0.75 ... More
No compensation in CD44 stem cell marker following BCL-2 suppression by antisense oligonucleotides.
Authors:Rubenstein M, Hollowell CM, Guinan P,
Journal:In Vivo
PubMed ID:23422486
'Antisense oligonucleotides have previously been used to target regulatory proteins in prostate cancer models. We evaluated mono- and bispecific oligonucleotides which comparably suppressed expression of B-cell lymphoma-2 (BCL-2) in LNCaP cells. Cells compensated by suppressing caspase-3 (an apoptosis promoter), and enhancing the expression of androgen receptor and co-activating p300 and ... More
Suppression of prolactin-induced signal transducer and activator of transcription 5b signaling and induction of suppressors of cytokine signaling messenger ribonucleic acid in the hypothalamic arcuate nucleus of the rat during late pregnancy and lactation.
Authors:Anderson GM, Beijer P, Bang AS, Fenwick MA, Bunn SJ, Grattan DR,
Journal:Endocrinology
PubMed ID:16857756
'During late pregnancy and lactation, the tuberoinfundibular dopamine (TIDA) neurons that regulate prolactin secretion by negative feedback become less able to produce dopamine in response to prolactin, leading to hyperprolactinemia. Because prolactin-induced activation of dopamine synthesis in these neurons requires the Janus kinase/signal transducer and activator of transcription 5b (STAT5b) ... More
Reduced myocilin expression in cultured monkey trabecular meshwork cells induced by a selective glucocorticoid receptor agonist: comparison with steroids.
Authors:Pfeffer BA, DeWitt CA, Salvador-Silva M, Cavet ME, López FJ, Ward KW,
Journal:Invest Ophthalmol Vis Sci
PubMed ID:19696178
'To assess in vitro myocilin (MYOC) expression in trabecular meshwork (TM) cells exposed to BOL-303242-X, a selective glucocorticoid receptor (GR) agonist (SEGRA), in comparison with dexamethasone (DEX), and prednisolone acetate (PA). After drug treatment of monkey TM cultures, MYOC protein in conditioned media (CM) was measured by Western blot and ... More