Rhod-2，AM，细胞可透过性
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Rhod-2，AM，细胞可透过性
引用和文献 (140)
Invitrogen™

Rhod-2，AM，细胞可透过性

标记的钙指示剂是结合 Ca2+ 后显示荧光增加的分子。它们可用于多种钙信号传导研究，包括自发荧光水平很高的细胞和组织中 Ca2+ 的测量以及光感受器和光活化螯合剂产生的 Ca2+ 释放的检测。将溶解后的指示剂直接加入含有培养细胞的培养皿中，即可向细胞上样 AM了解更多信息
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货号数量
R12441mg
R1245MP20 x 50 μg
货号 R1244
价格（CNY)
6,132.00
飞享价
Ends: 31-Dec-2025
8,258.00
共减 2,126.00 (26%)
Each
添加至购物车
数量:
1mg
价格（CNY)
6,132.00
飞享价
Ends: 31-Dec-2025
8,258.00
共减 2,126.00 (26%)
Each
添加至购物车
标记的钙指示剂是结合 Ca2+ 后显示荧光增加的分子。它们可用于多种钙信号传导研究，包括自发荧光水平很高的细胞和组织中 Ca2+ 的测量以及光感受器和光活化螯合剂产生的 Ca2+ 释放的检测。将溶解后的指示剂直接加入含有培养细胞的培养皿中，即可向细胞上样 AM 酯形式的这类钙离子指示剂。通常使用荧光显微镜检测这些细胞的荧光信号。

钙指示剂（AM 酯）规范：
•标签（Ca2+– 结合形式的激发/发射波长）：Rhod-2 (552/581 nm)
• 结合 Ca2+ 后荧光强度增加：>100 倍
•缓冲液中无 Mg2+ 时 Ca2+ 的 Kd：∼570 nM
• 结合 Ca2+ 后，荧光增加，波长稍有变化

使用 TPEN 控制重金属阳离子
此外，基于 BAPTA 的指示剂可结合各种重金属阳离子（例如 Mn2+、Zn2+、Pb+2），亲和力远高于 Ca2+。由于存在这些离子而引起的钙测量的扰动可以使用重金属选择性螯合剂 TPEN 进行控制。

钙荧光指示剂的更多选择
我们提供了大量 Molecular Probes™ 钙指示剂，可用于各种实验方案，例如右旋糖酐形式可减少泄漏和区室化、BAPTA 偶联物用于检测高幅钙瞬变。有关更多信息，请查看 Molecular Probes™ 手册中的使用可见光激发的荧光 Ca2+ 指示剂—第 19.3 节。

对于 UV 激发的 Ca2+ 指示剂、基于蛋白的 Ca2+ 指示剂、Ca2+ 指示剂的偶联物以及其他金属离子（即 Mg2+、Zn2+）的荧光指示剂，请查看 Molecular Probes™ 手册中的 Ca2+、Mg2+、Zn2+ 以及其他金属离子指示剂—第 19 章。

仅供科研使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型基于荧光染料
数量1mg
运输条件室温
适用于（应用）细胞活力与增殖
适用于（设备）荧光显微镜
产品类型染色剂
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the excitation/emission maxima for Rhod-2, AM, cell permeant (Cat. No. R1244, R1245MP)?

Rhod-2, AM, cell permeant (Cat. No. R1244, R1245MP) exhibits >100-fold increase in fluorescence intensity upon binding Ca2+. The excitation/emission maxima for Rhod-2, AM, cell permeant when bound to Ca2+ are 552/581 nm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (140)

引用和文献
Abstract
Functional implications of calcium permeability of the channel formed by pannexin 1.
Authors:Vanden Abeele F,Bidaux G,Gordienko D,Beck B,Panchin YV,Baranova AV,Ivanov DV,Skryma R,Prevarskaya N
Journal:The Journal of cell biology
PubMed ID:16908669
Although human pannexins (PanX) are homologous to gap junction molecules, their physiological function in vertebrates remains poorly understood. Our results demonstrate that overexpression of PanX1 results in the formation of Ca(2+)-permeable gap junction channels between adjacent cells, thus, allowing direct intercellular Ca(2+) diffusion and facilitating intercellular Ca(2+) wave propagation. More ... More
Spatially organised mitochondrial calcium uptake through a novel pathway in chick neurones.
Authors:Coatesworth W, Bolsover S
Journal:Cell Calcium
PubMed ID:16338004
'A brief depolarisation of chick sensory neurones evokes a calcium increase in mitochondria that peaks 1-2s after the depolarisation event and then decays over tens of seconds. Peripheral mitochondria take up more calcium than do central ones, even when the cytosolic calcium increase is spatially homogeneous. The calcium influx into ... More
Feedback inhibition of sodium/calcium exchange by mitochondrial calcium accumulation.
Authors:Opuni K, Reeves JP
Journal:J Biol Chem
PubMed ID:10801871
'Chinese hamster ovary cells expressing the bovine cardiac Na(+)/Ca(2+) exchanger were subjected to two periods of 5 and 3 min, respectively, during which the extracellular Na(+) concentration ([Na(+)](o)) was reduced to 20 mm; these intervals were separated by a 5-min recovery period at 140 mm Na(+)(o). The cytosolic Ca(2+) concentration ... More
Hydrolysis of Ca2+-sensitive fluorescent probes by perfused rat heart.
Authors:Scaduto RC, Grotyohann LW
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:14561682
'Rat hearts were loaded with the fluorescent calcium indicators fura 2, indo 1, rhod 2, or fluo 3 to determine cytosolic calcium levels in the perfused rat heart. With fura 2, however, basal tissue fluorescence increased above anticipated levels, suggesting accumulation of intermediates of fura 2-AM deesterification. To examine this ... More
Nitric oxide-dependent mitochondrial biogenesis generates Ca2+ signaling profile of lupus T cells.
Authors:Nagy G, Barcza M, Gonchoroff N, Phillips PE, Perl A
Journal:J Immunol
PubMed ID:15356113
'Abnormal T cell activation and cell death underlie the pathology of systemic lupus erythematosus. Although mitochondrial hyperpolarization (MHP) represents an early and reversible checkpoint of T cell activation and apoptosis, lupus T cells exhibit persistent MHP. NO has recently been recognized as a key signal of mitochondrial biogenesis and mediator ... More
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