Hoechst 33342 Ready Flow™ 即用型染料

引用和文献 (6)

Invitrogen™

Hoechst 33342 Ready Flow™ 即用型染料

Hoechst 33342 Ready Flow™ 即用型染料是一种明亮且易于使用的细胞渗透性染料，适用于使用流式细胞仪分析细胞周期。当该染料与双链 DNA 结合时，会发出蓝色荧光，最大发射波长为 461 nm了解更多信息

货号	数量
R37165	120 assays

货号 R37165

价格（CNY)

1,944.00

キャンペーン価格

Ends: 31-Dec-2026

2,582.00

共减 638.00 (25%)

添加至购物车

120 assays

价格（CNY)

1,944.00

キャンペーン価格

Ends: 31-Dec-2026

2,582.00

共减 638.00 (25%)

添加至购物车

Hoechst 33342 Ready Flow™ 即用型染料是一种明亮且易于使用的细胞渗透性染料，适用于使用流式细胞仪分析细胞周期。当该染料与双链 DNA 结合时，会发出蓝色荧光，最大发射波长为 461 nm。

•在室温下稳定
•方便、即用型—无需稀释、称重或移液
•具有细胞渗透性，可对固定细胞或活细胞高效染色

Hoechst 33342 Ready Flow 即用型染料是一种细胞渗透性的蓝色荧光 DNA 染料，用于细胞周期分析。从滴瓶中直接向 1 x 10⁶ 细胞中滴加 2 滴染料，孵育并分析。

Hoechst 33342 即用型染料优先与双链 DNA 的腺嘌呤-胸腺嘧啶区域结合。染料结合到 DNA 的小凹槽后，发射出明显的荧光光谱，荧光强度取决于染料：碱基对比例。Hoechst 33342 Ready Flow 即用型染料由紫外光激发，在 460 nm 处有发射峰。

染料类型细胞通透性

激发波长范围361/497

产品线Ready Flow

数量120 assays

产品类型核酸染色剂

亚细胞定位细胞核/核仁

Unit SizeEach

内容与储存

2 个滴瓶，在室温下储存

常见问题解答 (FAQ)

Is DAPI a good live-cell nuclear label?

DAPI is considered a semi-permeant/impermeant nucleic acid stain. Staining of nucleic is dependent upon the cell line in its performance. Some cell lines will label with DAPI, others not at all, and others label inconsistenly. Instead, we recommend using either Hoechst 33342 or Hoechst 33258, which have the same wavelength and binding mode as DAPI (at the A-T minor groove) but are readily cell-permeant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label the nuclei of live cells and track them over time. Can I use DAPI for this?

We do not recommend doing this. DAPI is considered to be a semi-permeant/impermeant nucleic acid stain. DAPI staining of live cells may be inconsistent. It is best used as a counterstain for fixed samples. Other cell permeable nucleic acid stains, such as Hoechst or the SYTO dyes may affect cellular function.

For mammalian cells, we recommend using the CellLight Nucleus transduction reagents, available in CFP, GFP and RFP. With these reagents, the cells are transduced overnight in a single labeling step and the next day the nuclei will fluoresce. The label may be retained for 3-5 days and should not affect cell function. Cytoplasmic cell tracking dyes such as the CellTracker dyes may also be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (6)

引用和文献

Abstract

Sphingosine-1-phosphate mediates the therapeutic effects of bone marrow mesenchymal stem cell-derived microvesicles on articular cartilage defect.

Authors:Xiang C, Yang K, Liang Z, Wan Y, Cheng Y, Ma D, Zhang H, Hou W, Fu P

Journal:Transl Res

PubMed ID:29324234

'Microvesicles (MVs) are emerging as a new mechanism of intercellular communication by transferring cellular components to target cells, yet their function in disease is just being explored. However, the therapeutic effects of MVs in cartilage injury and degeneration remain unknown. We found MVs contained high levels of sphingosine-1-phosphate (S1P) compared ... More

The Importance of Multifrequency Impedance Sensing of Endothelial Barrier Formation Using ECIS Technology for the Generation of a Strong and Durable Paracellular Barrier.

Authors:Robilliard LD, Kho DT, Johnson RH, Anchan A, O'Carroll SJ, Graham ES

Journal:Biosensors (Basel)

PubMed ID:29973526

'In this paper, we demonstrate the application of electrical cell-substrate impedance sensing (ECIS) technology for measuring differences in the formation of a strong and durable endothelial barrier model. In addition, we highlight the capacity of ECIS technology to model the parameters of the physical barrier associated with (I) the paracellular ... More

Diverse Signaling by TGFß Isoforms in Response to Focal Injury is Associated with Either Retinal Regeneration or Reactive Gliosis.

Authors:Conedera FM, Quintela Pousa AM, Presby DM, Mercader N, Enzmann V, Tschopp M

Journal:Cell Mol Neurobiol

PubMed ID:32219603

'Müller cells may have stem cell-like capability as they regenerate photoreceptor loss upon injury in some vertebrates, but not in mammals. Indeed, mammalian Müller cells undergo major cellular and molecular changes summarized as reactive gliosis. Transforming growth factor beta (TGFß) isoforms are multifunctional cytokines that play a central role, both ... More

ECIS technology reveals that monocytes isolated by CD14+ve selection mediate greater loss of BBB integrity than untouched monocytes, which occurs to a greater extent with IL-1ß activated endothelium in comparison to TNFa.

Authors:Kho DT, Johnson R, Robilliard L, du Mez E, McIntosh J, O'Carroll SJ, Angel CE, Graham ES

Journal:PLoS One

PubMed ID:28732059

We have previously shown that TNFa and IL-1ß differentially regulate the inflammatory phenotype of human brain endothelial cells (hCMVECs). In this regard, IL-1ß treatment was considerably more potent than TNFa at increasing expression of inflammatory chemokines and leukocyte adhesion molecules. We therefore hypothesised that interaction of the hCMVECs with human ... More

Colloid chemistry pitfall for flow cytometric enumeration of viruses in water.

Authors:Dlusskaya EA, Atrazhev AM, Ashbolt NJ

Journal:Water Res X

PubMed ID:31194069

Flow cytomtery (FCM) has become a standard approach to enumerate viruses in water research. However, the nature of the fluorescent signal in flow cytometric analysis of water samples and the mechanism of its formation, have not been addressed for bacteriophages expected in wastewaters. Here we assess the behaviour of fluorescent ... More

View all Hoechst 33342 Ready Flow™ 即用型染料 citations

Hoechst 33342 Ready Flow™ 即用型染料

常见问题解答 (FAQ)

Is DAPI a good live-cell nuclear label?

I want to label the nuclei of live cells and track them over time. Can I use DAPI for this?

I would like to label only 100 &micro;L of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

What is the concentration of dye/reagent in the Ready Flow products?

Can I use the ReadyProbes reagents for flow cytometry?

引用和文献 (6)

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?