CellEvent™ Caspase 3/7 Green Ready Flow™ 试剂
CellEvent™ Caspase 3/7 Green Ready Flow™ 试剂
引用和文献 (7)
Invitrogen™

CellEvent™ Caspase 3/7 Green Ready Flow™ 试剂

CellEvent™ Caspase-3/7 Green Ready Flow™ 试剂可用于流式细胞术检测凋亡细胞中活化的半胱天冬酶 3 和半胱天冬酶 7。该产品包括了解更多信息
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货号数量
R37167120 assays
货号 R37167
价格（CNY)
3,912.00
Online Exclusive
Ends: 31-Dec-2025
5,197.00
共减 1,285.00 (25%)
Each
添加至购物车
数量:
120 assays
价格（CNY)
3,912.00
Online Exclusive
Ends: 31-Dec-2025
5,197.00
共减 1,285.00 (25%)
Each
添加至购物车
CellEvent™ Caspase-3/7 Green Ready Flow™ 试剂可用于流式细胞术检测凋亡细胞中活化的半胱天冬酶 3 和半胱天冬酶 7。该产品包括 2 种试剂：新型荧光底物 CellEvent™ Caspase-3/7 Green 检测试剂和 SYTOX™ AADvanced™ 死细胞染色剂。

•可在室温下保持稳定
• 方便使用的即用型溶液—无需稀释、称重或者移取
• 与核酸结合后发出明亮的绿色荧光
• 无需洗涤步骤即可对凋亡细胞快速染色

CellEvent Caspase-3/7 Green Ready Flow 试剂是一种使用流式细胞分析检测半胱天冬酶 3/7 活性的细胞可透过性试剂。从滴瓶中直接向 1 x 106 个细胞中加入 2 滴试剂，然后孵育、分析。

CellEvent Caspase-3/7 Green Ready Flow 试剂由与核酸结合染料偶联的四氨基酸肽 (DEVD) 组成。细胞凋亡期间，caspase-3 和 caspase-7 蛋白激活，可切割 DEVD 多肽之中的编码 caspase 3/7 识别序列。识别序列断裂及试剂对 DNA 的结合可采用明亮的荧光信号（最大吸收/发射波长 ∼502/530 nm）标记凋亡细胞。当与 SYTOX AADvanced 死细胞染色剂一起使用时，很容易从活细胞和坏死细胞中区分出凋亡细胞。

由于在所有系统中没有单一的参数定义细胞凋亡，我们强烈建议使用不同的测量组合来可靠地检测细胞凋亡。
规格
颜色绿色
激发/发射504/523
标签类型其他标记或染料
标签或染料CellEvent™ 半胱天冬酶 3/7 Green、SYTOX AADvanced 死细胞染色剂
产品线CellEvent™ Ready Flow
产品类型半胱天冬酶 3/7 试剂
数量120 assays
运输条件室温
子细胞定位细胞核/核仁
产品规格滴瓶
Unit SizeEach
内容与储存
4个滴瓶，在室温下储存

常见问题解答 (FAQ)

我能固定我的CellEvent Caspase 3/7标记的细胞么？

可以，在3.7%甲醛中固定15分钟后，CellEvent信号将会保留。 

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use CellEvent Caspase-3/7 Green Detection Reagent with fixed samples?

No, it must be applied to live cell populations.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellEvent™ Caspase 3/7 Green Ready Flow™ 试剂 FAQs

引用和文献 (7)

引用和文献
Abstract
Alternatives to current flow cytometry data analysis for clinical and research studies.
Authors:Gondhalekar C, Rajwa B, Patsekin V, Ragheb K, Sturgis J, Robinson JP
Journal:Methods
PubMed ID:29305968
'Flow cytometry has well-established methods for data analysis based on traditional data collection techniques. These techniques typically involved manual insertion of tube samples into an instrument that, historically, could only measure 1-3 colors. The field has since evolved to incorporate new technologies for faster and highly automated sample preparation and ... More
Measurement and Characterization of Apoptosis by Flow Cytometry.
Authors:Telford W, Tamul K, Bradford J
Journal:Curr Protoc Cytom
PubMed ID:27367289
'Apoptosis is an important mechanism in cell biology, playing a critical regulatory role in virtually every organ system. It has been particularly well characterized in the immune system, with roles ranging from immature immune cell development and selection to down-regulation of the mature immune response. Apoptosis is also the primary ... More
Computational flow cytometry reveals that cryopreservation induces spermptosis but subpopulations of spermatozoa may experience capacitation-like changes.
Authors:Ortega-Ferrusola C, Anel-López L, Martín-Muñoz P, Ortíz-Rodríguez JM, Gil MC, Alvarez M, de Paz P, Ezquerra LJ, Masot AJ, Redondo E, Anel L, Peña FJ
Journal:Reproduction
PubMed ID:27965398
'The reduced lifespan of cryopreserved spermatozoa in the mare reproductive tract has been attributed to both capacitative and apoptotic changes. However, there is a lack of studies investigating both phenomena simultaneously. In order to improve our knowledge in this particular point, we studied in raw and frozen-thawed samples apoptotic and ... More
Effects of ghrelin on the apoptosis of human neutrophils in vitro.
Authors:Li B, Zeng M, Zheng H, Huang C, He W, Lu G, Li X, Chen Y, Xie R
Journal:Int J Mol Med
PubMed ID:27431014
Acute respiratory distress syndrome (ARDS) is characterized by lung inflammation and the diffuse infiltration of neutrophils into the alveolar space. Neutrophils are abundant, short-lived leukocytes that play a key role in immune defense against microbial infections. These cells die via apoptosis following the activation and uptake of microbes, and will also ... More
New flow cytometry approaches in equine andrology.
Authors:Peña FJ, Ortega Ferrusola C, Martín Muñoz P
Journal:Theriogenology
PubMed ID:27160445
Flow cytometry is currently recognized as a robust tool for the evaluation of sperm quality and function. However, within equine reproduction, this technique has not reached the sophistication of other areas of biology and medicine. In recent years, more sophisticated flow cytometers have been introduced in andrology laboratories, and the ... More
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