Schneider 培养基中的果蝇 S2 细胞
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引用和文献 (4)
Gibco™

Schneider 培养基中的果蝇 S2 细胞

Gibco ™果蝇 S2 细胞用于在 DrosophilaM 表达系统 (DES™) 中进行异源蛋白表达。S2 细胞系源自黑腹果蝇胚胎晚期了解更多信息
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货号数量
R690071 mL
货号 R69007
价格（CNY)
7,762.80
飞享价
Ends: 31-Dec-2025
9,224.00
共减 1,461.20 (16%)
Each
添加至购物车
数量:
1 mL
价格（CNY)
7,762.80
飞享价
Ends: 31-Dec-2025
9,224.00
共减 1,461.20 (16%)
Each
添加至购物车
Gibco ™果蝇 S2 细胞用于在 DrosophilaM 表达系统 (DES™) 中进行异源蛋白表达。S2 细胞系源自黑腹果蝇胚胎晚期（20-24 小时龄）的原代培养物。S2 细胞系的许多特点表明其来源于巨噬细胞样谱系。S2 细胞在室温下于环境 CO2 中在组织培养瓶中生长为松散的半贴壁单层细胞，然后在旋转器和摇瓶中悬浮培养。果蝇 S2 细胞（在 Gibco™ Schneider 培养基中冷冻）的特点：
•可瞬时或通过生成稳定细胞系表达蛋白
• 质量和性能检测

可瞬时或通过生成稳定细胞系表达蛋白
果蝇 S2 细胞可单独使用重组表达载体转染，用于瞬时表达研究，或者与选择载体联合使用（例如 pCoHygro 或 pCoBlast），用于生成稳定细胞系。提供多种 DES™ 试剂盒以便在 S2 细胞中表达目标重组蛋白。

质量和性能检测
对每个批次的 Gibco™ S2 细胞进行细胞生长以及低温贮藏回收后细胞存活率检测。此外，已对主种子库的细菌、酵母菌、支原体和病毒的污染进行了检测，并已通过同工酶和核型分析进行了表征。

注意：请至少于2级生物安全防护措施条件下操作任何具有潜在生物危害性的材料。本品含有二甲亚砜（DMSO），这是一种有害试材。请在操作前浏览材料安全性数据表。请在操作前浏览材料安全性数据表。
仅供科研使用。不可用于人或任何动物的治疗或诊断用途。
规格
产品类型果蝇 S2 细胞
数量1 mL
细胞系S2 (Schneider 2)
细胞类型昆虫细胞
种属果蝇
Unit SizeEach
内容与储存
储存条件：液氮（气相）
运输条件：冰冻

常见问题解答 (FAQ)

What is the doubling time of Drosophila S2 cells?

When grown at the optimal temperature of 27-28ºC, Drosophila S2 cells grow very quickly, with an approximate 14-16 hr doubling time. The cells can also be grown at 22-25ºC with doubling time of 24hrs. Humidity is an issue, so a shallow pan of water can be left in the incubator, but the incubator does not need to be turned on.

Growth medium is also a factor in doubling time - the doubling times listed here are for Sf900 II medium.

The cells can reach maximum densities of 1 to 2.5 x 10E7 cells/ml in shake flask cultures.

I am working with S2 cells that my lab has purchased from you. Can you please let me know which solvent I should use to dissolve the CuSO4?

CuSO4 is water soluble. You can dissolve it in water for cell culture use, and sterile-filter it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用和文献 (4)

引用和文献
Abstract
Inhibitory Potency and Specificity of Subtilase-like Pro-protein Convertase (SPC) Prodomains.
Authors: Fugere Martin; Limperis Polizois C; Beaulieu-Audy Veronique; Gagnon Frederic; Lavigne Pierre; Klarskov Klaus; Leduc Richard; Day Robert;
Journal:J Biol Chem
PubMed ID:11723118
'The SPCs (subtilisin-like pro-protein convertases) are a family of enzymes responsible for the proteolytic processing of numerous precursor proteins of the constitutive and regulated secretory pathways. SPCs are themselves synthesized as inactive zymogens. Activation of SPCs occurs via the intramolecular autocatalytic removal of the prodomain. SPC prodomains have been proposed ... More
The human cytomegalovirus immediate early gene promoter is a strong promoter in cultured Drosophila melanogaster cells.
Authors: Sinclair J H;
Journal:Nucleic Acids Res
PubMed ID:3031595
None
A heterotrimeric death domain complex in Toll signaling.
Authors: Sun Huaiyu; Bristow Benjamin N; Qu Guowei; Wasserman Steven A;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12351681
Signaling from the transmembrane receptor Toll to Rel-related transcription factors regulates dorsoventral patterning of the Drosophila embryo, as well as larval and adult immunity. To identify additional pathway components, we have used double-stranded RNA interference to investigate Drosophila counterparts of genes that regulate the mammalian Rel family member NF-kappaB. Experiments ... More
Drosophila Ack targets its substrate, the sorting nexin DSH3PX1, to a protein complex involved in axonal guidance.
Authors: Worby Carolyn A; Simonson-Leff Nancy; Clemens James C; Huddler Donald Jr; Muda Marco; Dixon Jack E;
Journal:J Biol Chem
PubMed ID:11773052
Dock, the Drosophila orthologue of Nck, is an adaptor protein that is known to function in axonal guidance paradigms in the fly including proper development of neuronal connections in photoreceptor cells and axonal tracking in Bolwig's organ. To develop a better understanding of axonal guidance at the molecular level, we ... More