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引用和文献 (17)
Invitrogen™
ProBond™ 镍螯合树脂
ProBond™ 镍螯合树脂是一种镍荷电亲和树脂，用于纯化含多聚组氨酸 (6xHis) 序列的重组蛋白。与树脂结合的蛋白可使用低 pH 值缓冲液或通过与咪唑或组氨酸竞争进行洗脱。在天然和变性条件下均可进行一步纯化。ProBond™ 树脂使用螯合配体亚氨基二乙酸了解更多信息
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| 货号 | 数量 |
|---|---|
| R80101 | 50 mL |
| R80115 | 150 mL |
| R80115TS 又称 R80115 |
货号 R80101
价格（CNY)
11,876.00
Each
数量:
50 mL
价格（CNY)
11,876.00
Each
ProBond™ 镍螯合树脂是一种镍荷电亲和树脂，用于纯化含多聚组氨酸 (6xHis) 序列的重组蛋白。与树脂结合的蛋白可使用低 pH 值缓冲液或通过与咪唑或组氨酸竞争进行洗脱。在天然和变性条件下均可进行一步纯化。ProBond™ 树脂使用螯合配体亚氨基二乙酸 (IDA)，该配体偶联至适用于 FPLC、批次和重力流应用的高度交联 6% 琼脂糖树脂。
仅供科研使用。不可用于诊断程序。
规格
数量50 mL
固定相镍螯合
形式悬浮
产品线ProBond
类型树脂
Unit SizeEach
内容与储存
预先装入 ProBond™ 树脂，每 1 mL 树脂能够结合 1-5 mg 重组蛋白。其以含 50% 浆液的 20% 乙醇溶液的形式提供。带有双价镍电荷 (Ni2+) 时，树脂将呈蓝色。在 +4°C 下储存。妥善储存时，保证 ProBond™ 树脂稳定储存 6 个月。
常见问题解答 (FAQ)
你们通常怎样检测重组融合蛋白的表达？
How do you typically detect expression of a recombinant fusion protein?
What expression levels can be expected with the pTrcHis/CAT construct?
I purified my protein from a ProBond column using denaturing conditions. After elution, I tried digesting off my N- terminal tag with EKMax Enterokinase, but see no EK cleavage. What can you suggest I try?
Can ProBond or Ni-NTA beads be used for large-scale preparations?
引用和文献 (17)
引用和文献
Abstract
Total synthesis of cyclic ADP-carbocyclic-ribose, a stable mimic of Ca2+-mobilizing second messenger cyclic ADP-ribose.
Journal:J Am Chem Soc
PubMed ID:11535079
'The synthesis of cyclic ADP-carbocyclic-ribose (cADPcR, 4) designed as a stable mimic of cyclic ADP-ribose (cADPR, 1), a Ca2+-mobilizing second messenger, was achieved using as the key step a condensation reaction with the phenylthiophosphate-type substrate 14 to form an intramolecular pyrophosphate linkage. The N-1-carbocyclic-ribosyladenosine derivative 16 was prepared via the
Thr-161 Phosphorylation of Monomeric Cdc2. REGULATION BY PROTEIN PHOSPHATASE 2C IN XENOPUS OOCYTES.
Journal:J Biol Chem
PubMed ID:12036957
'Fully grown Xenopus oocyte is arrested at prophase I of meiosis. Re-entry into meiosis depends on the activation of MPF (M-phase promoting factor or cyclin B.Cdc2 complex), triggered by progesterone. The prophase-arrested oocyte contains a store of Cdc2. Most of the protein is present as a monomer whereas a minor
Neutrophil phospholipase D is activated by a membrane-associated Rho family small molecular weight GTP-binding protein.
Journal:J Biol Chem
PubMed ID:8408000
'Phospholipase D in human neutrophil lysates is activated by GTP gamma S (guanosine 5''-O-(3-thiotriphosphate)), implying the participation of a GTP-binding protein. Reconstitution of GTP gamma S-dependent activity requires protein factors in both the plasma membrane and the cytosol (Olson, S. C., Bowman, E. P., and Lambeth, J. D. (1991) J.
The plasmid RK2 initiation protein binds to the origin of replication as a monomer.
Journal:J Biol Chem
PubMed ID:8636140
'The TrfA protein encoded by the broad host range bacterial plasmid RK2 specifically binds to eight direct repeats (iterons) present at the plasmid replication origin to initiate DNA replication. Purified TrfA protein is largely in the form of a dimer, and using a dimerization test system that involves the fusion
Saccharomyces cerevisiae cytoplasmic tyrosyl-tRNA synthetase gene. Isolation by complementation of a mutant Escherichia coli suppressor tRNA defective in aminoacylation and sequence analysis.
Journal:J Biol Chem
PubMed ID:8509419
'Exploiting differences in tRNA recognition between prokaryotic and eukaryotic tyrosyl-tRNA synthetases (TyrRSs), we have isolated the gene for the cytoplasmic TyrRS of Saccharomyces cerevisiae by functional complementation in Escherichia coli of a mutant E. coli tRNA. The tRNA, derived from the E. coli initiator tRNA with changes to allow suppression