SiteClick™ R-PE 抗体标记试剂盒
SiteClick™ R-PE 抗体标记试剂盒
Invitrogen™

SiteClick™ R-PE 抗体标记试剂盒

使用 SiteClick™ R-PE 抗体标记试剂盒创建一个完全标记抗体。与传统的抗体标记试剂盒不同,SiteClick™ 标记可以特异性地将标记连接至 IgG 抗体的重链上,确保抗原结合结构域仍可与抗原靶标结合。该位点选择性是通过靶向基本上所有 IgG了解更多信息
Have Questions?
货号 S10467
价格(CNY)
7,080.00
Each
添加至购物车
价格(CNY)
7,080.00
Each
添加至购物车
使用 SiteClick™ R-PE 抗体标记试剂盒创建一个完全标记抗体。与传统的抗体标记试剂盒不同,SiteClick™ 标记可以特异性地将标记连接至 IgG 抗体的重链上,确保抗原结合结构域仍可与抗原靶标结合。该位点选择性是通过靶向基本上所有 IgG 抗体(不考虑同种型和宿主种类)上存在的碳水化合物结构域而实现的。根据标记,得到的 SiteClick™ 标记抗体可用于流式细胞分析或任何其他应用。

SiteClick™ R-PE 抗体标记试剂盒的重要特点:

•包含标记 100 μg IgG 抗体所需的一切成分
•易于遵循逐步方案
•高效、位点特异性、可重现的标记化学产生高质量抗体偶联物。

R-PE(R-藻红蛋白)为明亮标记
R-藻红蛋白 (R-PE) 是一种从红藻中提取的明亮藻胆蛋白。其一级吸收(激发)峰在 565 nm 处,二级峰在 496 和 545 nm 处。宽激发光谱和极明亮的荧光(最大发射波长 = 约 578 nm)使其成为免疫细胞化学(尤其是流式细胞分析和细胞分选 (FACS))应用中多通路技术的有用标记。

了解更多关于蛋白和抗体标记的信息
我们可提供多种可供选择的 Molecular Probes® 抗体和蛋白标记试剂盒(参见 A 至 Z 的抗体标记)。要了解更多关于不同试剂盒的信息,请阅读 Molecular Probes® 手册中标记蛋白和核酸的试剂盒—第 1.2 节

定制 SiteClick™ 抗体标记服务
如果您有一种被视为“难以标记”或在使用传统方法标记后失去活性的抗体,请联系我们的定制服务代表,以确定 SiteClick™ 抗体标记服务是否适用于您的抗体。我们提供完整的定制 SiteClick™ 抗体标记服务,有多种检测分子可供选择,包括生物素、Alexa Fluor® 染料、Qdot® 荧光基团、R-PE 和许多其他选择。
仅供科研使用。不可用于诊断程序。
规格
颜色橙红色
激发/发射496、546、565/578
流式细胞仪激光线路蓝色、绿色、黄色
套装内容包含叠氮基修饰和标记 100 µg 抗体所需的一切组分。
标记方法SiteClick 标记系统
标记规模100 μg
产品线SiteClick
产品类型Labeling Kit
数量1 kit
运输条件湿冰
标记目标IgG
标签或染料R-PE(R-藻红蛋白)
Unit SizeEach
内容与储存
接收后将整个试剂盒储存于 2 至 6°C 下。

常见问题解答 (FAQ)

I need to label my tissue section with two mouse primary antibodies. Do I have to make direct conjugates of the antibodies to use them?

Check the isotype of the two antibodies to see if they are different. If so, we offer isotype-specific goat anti-mouse secondary antibodies that can be used together with minimal cross-reactivity. If the two mouse antibodies are of the same isotype, then you would need to make direct conjugates, such as with our antibody labeling kits or APEX, and SiteClick kits.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (4)

引用和文献
Abstract
Enzyme-mediated methodology for the site-specific radiolabeling of antibodies based on catalyst-free click chemistry.
Authors:Zeglis BM, Davis CB, Aggeler R, Kang HC, Chen A, Agnew BJ, Lewis JS,
Journal:
PubMed ID:23688208
'An enzyme- and click chemistry-mediated methodology for the site-selective radiolabeling of antibodies on the heavy chain glycans has been developed and validated. To this end, a model system based on the prostate specific membrane antigen-targeting antibody J591, the positron-emitting radiometal (89)Zr, and the chelator desferrioxamine has been employed. The methodology ... More
Targeting the CXCR4 pathway using a novel anti-CXCR4 IgG1 antibody (PF-06747143) in chronic lymphocytic leukemia.
Authors:Kashyap MK, Amaya-Chanaga CI, Kumar D, Simmons B, Huser N, Gu Y, Hallin M, Lindquist K, Yafawi R, Choi MY, Amine AA, Rassenti LZ, Zhang C, Liu SH, Smeal T, Fantin VR, Kipps TJ, Pernasetti F, Castro JE
Journal:J Hematol Oncol
PubMed ID:28526063
'The CXCR4-CXCL12 axis plays an important role in the chronic lymphocytic leukemia (CLL)-microenvironment interaction. Overexpression of CXCR4 has been reported in different hematological malignancies including CLL. Binding of the pro-survival chemokine CXCL12 with its cognate receptor CXCR4 induces cell migration. CXCL12/CXCR4 signaling axis promotes cell survival and proliferation and may ... More
Actin-Related Protein 2 (ARP2) and Virus-Induced Filopodia Facilitate Human Respiratory Syncytial Virus Spread.
Authors:Mehedi M, McCarty T, Martin SE, Le Nouën C, Buehler E, Chen YC, Smelkinson M, Ganesan S, Fischer ER, Brock LG, Liang B, Munir S, Collins PL, Buchholz UJ
Journal:PLoS Pathog
PubMed ID:27926942
Human respiratory syncytial virus (RSV) is an enveloped RNA virus that is the most important viral cause of acute pediatric lower respiratory tract illness worldwide, and lacks a vaccine or effective antiviral drug. The involvement of host factors in the RSV replicative cycle remains poorly characterized. A genome-wide siRNA screen ... More
Donor-derived cell-free DNA detects kidney transplant rejection during nivolumab treatment.
Authors:Hurkmans DP, Verhoeven JGHP, de Leur K, Boer K, Joosse A, Baan CC, von der Thüsen JH, van Schaik RHN, Mathijssen RHJ, van der Veldt AAM, Hesselink DA
Journal:J Immunother Cancer
PubMed ID:31300068
In solid organ transplant (SOT) recipients, transplant rejection during immune checkpoint inhibitor (ICI) treatment for cancer is a clinical problem. Donor-derived cell-free DNA (dd-cfDNA) can be detected in blood and is a sensitive biomarker for diagnosis of acute rejection in SOT recipients. To our best knowledge, this is the first ... More