Search
引用和文献 (88)
Invitrogen™
链霉亲和素-别藻蓝蛋白偶联物，优质级
链霉亲和素-别藻蓝蛋白偶联物，优质级
| 货号 | 数量 |
|---|---|
| S32362 又称 S-32362 | 250 μL |
货号 S32362
又称 S-32362
价格（CNY)
12,472.00
Each
数量:
250 μL
价格（CNY)
12,472.00
Each
Streptavidin is a bacterially derived biotin-binding protein that reportedly exhibits less nonspecific binding than does avidin. Allophycocyanin is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ∼650 nm, and a fluorescence emission maximum of ∼660 nm. Our streptavidin allophycocyanin conjugate *premium grade* is a further fractionation of our S-868 product, intended especially for applications that benefit from additional purity.
Related Links:
链霉亲和素是一种细菌来源的生物素结合蛋白，据报告非特异性结合比例低于亲和素。别藻蓝蛋白是一种非常明亮的藻胆蛋白，具有较宽的吸收光谱（峰值在 ∼650 nm 处），最大荧光发射波长为 ∼660 nm。我们的链霉素亲和素别藻蓝蛋白偶联物*（优质级）*由我们的 S-868 产品进一步分离所得，特别适合需要额外纯度的应用。
查看所有链霉素亲和素 RPE、APC 以及串联偶联物选择指南。
查看所有链霉素亲和素 RPE、APC 以及串联偶联物选择指南。
仅供科研使用。不可用于诊断程序。
规格
最大浓度1 mg/mL
检测方法荧光
激发/发射650/660
流式细胞仪激光线路633 或 635
适用于（设备）流式细胞分析、免疫检测、组化应用、印迹分析
标签或染料APC(别藻蓝素)
产品类型链霉素亲和素偶联物(荧光)
数量250 μL
运输条件湿冰
偶联物APC
形式溶液
Unit SizeEach
内容与储存
包含1小瓶优质级链霉亲和素 APC 偶联物 (1 mg/mL)。
请避光冷藏 (2–8°C) 储存。
请避光冷藏 (2–8°C) 储存。
常见问题解答 (FAQ)
For Streptavidin, Allophycocyanin Conjugate, premium grade (Cat. No. S32362), how many molecules of APC are conjugated to each streptavidin molecule?
How should I store my allophycocyanin (APC)-conjugated antibody?
引用和文献 (88)
引用和文献
Abstract
Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers.
Journal:Cytometry A
PubMed ID:18836993
'The ability to quantify and characterize antigen-specific CD8+ T cells irrespective of functional readouts using fluorochrome-conjugated peptide-major histocompatibility complex class I (pMHCI) tetramers in conjunction with flow cytometry has transformed our understanding of cellular immune responses over the past decade. In the case of prevalent CD8+ T cell populations that
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.
Journal:Anal Biochem
PubMed ID:12419349
'The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the biotin/biotin-binding protein interaction is that it is essentially irreversible under physiological conditions. Desthiobiotin is a biotin analogue that binds less tightly to biotin-binding proteins and is easily displaced by
Nonradioactive methods for the assay of phosphoinositide 3-kinases and phosphoinositide phosphatases and selective detection of signaling lipids in cell and tissue extracts.
Journal:Anal Biochem
PubMed ID:12605860
'We describe a novel approach to quantitation of phosphoinositides in cell extracts and in vitro enzyme-catalyzed reactions using suitably tagged and/or labeled pleckstrin homology (PH) domains as probes. Stable complexes were formed between the biotinylated target lipid and an appropriate PH domain, and phosphoinositides present in samples were detected by
Development of a ubiquitin transfer assay for high throughput screening by fluorescence resonance energy transfer.
Journal:J Biomol Screen
PubMed ID:11080690
'An assay based on fluorescence resonance energy transfer (FRET) has been developed to screen for ubiquitination inhibitors. The assay measures the transfer of ubiquitin from Ubc4 to HECT protein Rsc 1083. Secondary reagents (streptavidin and antibody to glutathione-S-transferase [GST]), pre-labeled with fluorophores (europium chelate, Eu(3+), and allophycocyanin [APC]), are noncovalently
Cell surface markers in human embryonic stem cells.
Journal:Methods Mol Biol
PubMed ID:18453248
'The pluripotent nature of human embryonic stem cells (hESCs) is based on their potential to form every cell type in the body. Prior to use in directed differentiation strategies, these cells need to be thoroughly characterized. The large number of glycoproteins and carbohydrates that exist on the cell surface provide