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SlowFade™ Glass 软质抗淬灭封片剂，含 DAPI
引用和文献 (1)
Invitrogen™

SlowFade™ Glass 软质抗淬灭封片剂，含 DAPI

SlowFade Glass 抗淬灭封片剂是一种基于甘油的即用型非固化封片剂，直接应用于显微镜玻片上的荧光标记细胞或组织样品。其配制为具有 1.52 的折射率，以获得更清晰的图像和抗淬灭组分，以抗光漂白。结果是使用焦点深度为 0–500 µm 的宽视野了解更多信息
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货号数量
S369201 x 2 mL
S36920-5X2ML5 x 2 mL
货号 S36920
价格（CNY)
939.00
Each
数量:
1 x 2 mL
价格（CNY)
939.00
Each

SlowFade Glass 抗淬灭封片剂是一种基于甘油的即用型非固化封片剂，直接应用于显微镜玻片上的荧光标记细胞或组织样品。其配制为具有 1.52 的折射率，以获得更清晰的图像和抗淬灭组分，以抗光漂白。结果是使用焦点深度为 0–500 µm 的宽视野、共焦或超分辨率显微镜获得几乎任何荧光基团的清晰明亮图像。

由于 SlowFade Glass 抗淬灭封片剂为非固化，可以在封片后立即对细胞进行成像，而不会对细胞形态产生任何不利影响，而固化封片剂可能引起这些不良影响。即用型—只需滴入样品，盖上盖玻片，并成像。含或不含 DAPI 细胞核染色剂。

SlowFade Glass 抗淬灭封片剂的特点包括：
•即用型、基于甘油的非固化抗淬灭封片剂
• 折射率为 1.52，尽可能减少球面像差
• 轴向分辨率为常规封片剂的三倍
•可见组织光学透明化，甚至在 1 mm 厚的切片中亦是如此
• 在0 至 500 µm 的任何焦点深度下均可获得清晰明亮的图像

用于深度 3D 成像的清晰图像
SlowFade Glass 抗淬灭封片剂的折射率为 1.52（与玻璃盖玻片相似），具有优异的光漂白保护。它与浸油和油浸显微镜光学元件兼容，可实现无折射光路并尽可能减少球面像差，通过点扩散函数测量，其可将轴向分辨率提高三倍（在 150 µm 焦点深度处）。当对 1 mm 厚组织切片封片时，存在可见的光学组织透明化，从而实现深度 3D 成像。

抑制可见光和近红外光谱的光漂白
无论您使用的是哪种荧光染料或荧光蛋白，Slowfade Glass 抗淬灭封片剂都有助于提供卓越的光漂白保护。经证实其在一系列荧光染料（包括 Alexa Fluor、FITC、TRTC、Texas Red、Cy3 和 Cy5 染料）和荧光蛋白（包括 GFP、RFP 和 mCherry）中具有极佳性能。SlowFade Glass 封片剂不会淬灭荧光基团，可以使荧光染料在无背景信号的情况下发出更明亮的信号，有助于您获得极高质量、高分辨率的图像，可供发表使用。

非固化封片剂不会使标本膨胀或收缩
大多数组织透明化技术都会使标本膨胀或收缩，导致标本形态失真。SlowFade Glass 抗淬灭封片剂为非固化，不会导致标本膨胀或收缩，我们已利用 Hela 球体和大鼠脑切片证实了这一点。这种特征可实现对真正天然的标本形态进行 3D 成像。标本在封片后立即可用于成像，适当密封的载玻片可储存数周或数月以获得优异的成像结果

仅供科研使用。不可用于诊断程序。
规格
数量1 x 2 mL
运输条件经批准可置于湿冰或干冰上运输
产品线SlowFade
产品类型抗淬灭固定剂
试剂类型抗褪色溶液
容积（公制）2 mL
Unit SizeEach
内容与储存
含 1 个滴瓶，滴瓶含 2 mL 试剂

常见问题解答 (FAQ)

I do not have epoxy or VALAP to seal the coverslip. Do you offer an alternative coverslip sealant?

We offer ProLong Coverslip Sealant (Cat. No. P56128) that can be used to seal the edges of the coverslip and is compatible with both curing and non-curing mountant. The sealant is easy to apply and is brushed on after the mountant has cured, for long preservation of slides. The product page can be found here.

When using a hard-curing mountant, such as ProLong Antifade Mountant, make sure the mountant is fully cured before applying ProLong Coverslip Sealant. Since this sealant can seal moisture under the coverslip, it can interfere with the curing process.

When using a non-curing mountant, such as SlowFade Antifade Mountants, sealing can take place immediately after mounting.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Will SlowFade Glass Soft-set Antifade Mountant, with DAPI (Cat. No. S36920), preserve the structure of fluorescent proteins?

No. SlowFade Glass Soft-set Antifade Mountant with DAPI (Cat. No. S36920) does not maintain the structures of fluorescent proteins over time. The proteins will naturally unfold, and this unfolding would result in the loss of fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What can be done to remove any tiny air bubbles in my SlowFade Glass Soft-set Antifade Mountant, with DAPI (Cat. No. S36920)?

To remove any tiny air bubbles, place the vial of SlowFade Glass Soft-set Antifade Mountant, with DAPI (Cat. No. S36920), within a vacuum chamber with the vial cap loosened. Degas the solution by applying vacuum through either a T-connector on the faucet or a very brief application of vacuum from a vacuum pump.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does SlowFade Glass Soft-set Antifade Mountant, with DAPI (Cat. No. S36920), contain any volatile organic solvents?

No. SlowFade Glass Soft-set Antifade Mountant, with DAPI (Cat. No. S36920), is an aqueous-based mountant.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Does Slowfade Antifade Mountant require using a nail polish for sealing?

No. Samples mounted with SlowFade mountants need not be sealed; they are intended for immediate viewing. The coverslip may be anchored (to prevent movement while viewing) by applying molten paraffin to three or four spots around the edge of the coverslip.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (1)

引用和文献
Abstract
4'-C-Trifluoromethyl modified oligodeoxynucleotides: synthesis, biochemical studies, and cellular uptake properties.
Authors:Zhou Y, Zang C, Wang H, Li J, Cui Z, Li Q, Guo F, Yan Z, Wen X, Xi Z, Zhou C
Journal:Org Biomol Chem
PubMed ID:31112186
Herein, we report the synthesis of 4'-C-trifluoromethyl (4'-CF3) thymidine (T4'-CF3) and its incorporation into oligodeoxynucleotides (ODNs) through solid-supported DNA synthesis. The 4'-CF3 modification leads to a marginal effect on the deoxyribose conformation and a local helical structure perturbation for ODN/RNA duplexes. This type of modification slightly decreases the thermal stability ... More