链霉亲和素-β-半乳糖苷酶偶联物
链霉亲和素-β-半乳糖苷酶偶联物
引用和文献 (5)
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链霉亲和素-β-半乳糖苷酶偶联物

链霉亲和素-ß-半乳糖苷酶偶联物可配合显色底物或荧光底物使用，在信号扩增方案中检测生物素。了解更多信息
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货号数量
S931
又称 S-931
1 mg
货号 S931
又称 S-931
价格（CNY)
5,670.00
Each
添加至购物车
数量:
1 mg
价格（CNY)
5,670.00
Each
添加至购物车
链霉亲和素-ß-半乳糖苷酶偶联物可配合显色底物或荧光底物使用，在信号扩增方案中检测生物素。
仅供科研使用。不可用于诊断程序。
规格
产品类型链霉素亲和素偶联物(荧光)
数量1 mg
运输条件湿冰
偶联物β-半乳糖苷酶
形式实心
Unit SizeEach
内容与储存
在冰箱(-5 至 -30°C)中储存。

引用和文献 (5)

引用和文献
Abstract
Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays.
Authors:Bobrow MN, Harris TD, Shaughnessy KJ, Litt GJ
Journal:J Immunol Methods
PubMed ID:2558138
'A novel signal amplification method, catalyzed reporter deposition (CARD), and its application to immunoassays is described. The method involves utilizing an analyte-dependent reporter enzyme (ADRE) to catalyze the deposition of additional reporter on the surface in a solid-phase immunoassay. In the examples described, deposition of reporter is facilitated by using ... More
Fluorescence probe studies of the state of tropomyosin in reconstituted muscle thin filaments.
Authors:Ishii Y, Lehrer SS
Journal:Biochemistry
PubMed ID:3663633
'The monomer fluorescence of N-(1-pyrenyl)maleimide-labeled tropomyosin bound to F-actin (PTm-actin) increases when myosin subfragment 1 (S1) binds to actin and is half complete when only approximately 1 S1 is bound to 7 actin subunits [Ishii, Y., & Lehrer, S. S. (1985) Biochemistry 24, 6631-6638]. Similar studies of the binding of ... More
Fluorescence sandwich enzyme-linked immunosorbent assay for detecting human interleukin-2 receptors.
Authors:Honda M, Nagao S, Yamamoto N, Tanaka Y, Tozawa H, Tokunaga T
Journal:J Immunol Methods
PubMed ID:2453583
A very sensitive fluorescence sandwich enzyme-linked immunosorbent assay (FS-ELISA) for the detection of soluble and cell-associated human interleukin-2 receptors (IL-2R) has been developed. For use in this assay system, two anti-human IL-2R monoclonal antibodies, H 48 and biotinylated HA 26, were selected from six monoclonal antibodies directed against different epitopes ... More
Measurement of antigen-specific mouse IgE by a fluorometric reverse (IgE-capture) ELISA.
Authors:Sakaguchi M, Inouye S, Miyazawa H, Tamura S
Journal:J Immunol Methods
PubMed ID:2783445
A reverse, or IgE-capture, enzyme-linked immunosorbent assay (ELISA) for measuring ovalbumin-specific IgE antibody in the serum of immunized mice has been developed. Microplate wells were first coated with a commercial anti-mouse IgE rat monoclonal antibody, and then incubated with two-fold serial dilutions of test sera with 10% normal mouse serum ... More
TNF-alpha pretreatment prevents subsequent activation of cultured brain cells with TNF-alpha and hypoxia via ceramide.
Authors:Ginis I, Schweizer U, Brenner M, Liu J, Azzam N, Spatz M, Hallenbeck JM
Journal:Am J Physiol
PubMed ID:10329967
We have developed a cellular model in which cultured astrocytes and brain capillary endothelial cells preconditioned with tumor necrosis factor-alpha (TNF-alpha) fail to upregulate intercellular adhesion molecule-1 (ICAM-1) protein (80% inhibition) and mRNA (30% inhibition) when challenged with TNF-alpha or exposed to hypoxia. Inasmuch as ceramide is known to mediate ... More