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引用和文献 (57)
Invitrogen™
链霉素亲和素琼脂糖、沉淀微珠悬浮液
与 4% 微珠状交联琼脂糖偶联的链霉素亲和素可用于分离生物素化蛋白。此外，生物素化抗体可以与链霉素亲和素琼脂糖结合，产生亲和介质用于抗原的大规模分离。了解更多信息
| 货号 | 数量 |
|---|---|
| S951 | 5 mL |
货号 S951
价格（CNY)
5,932.00
Each
数量:
5 mL
价格（CNY)
5,932.00
Each
与 4% 微珠状交联琼脂糖偶联的链霉素亲和素可用于分离生物素化蛋白。此外，生物素化抗体可以与链霉素亲和素琼脂糖结合，产生亲和介质用于抗原的大规模分离。
仅供科研使用。不可用于诊断程序。
规格
色谱柱类型亲和
标签或染料琼脂糖
数量5 mL
运输条件湿冰
固定相链霉亲和素
形式悬浮液
类型琼脂糖
Unit SizeEach
内容与储存
储存在冰箱 (2–8°C) 中。
常见问题解答 (FAQ)
What is the binding capacity of streptavidin agarose?
引用和文献 (57)
引用和文献
Abstract
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.
Journal:Anal Biochem
PubMed ID:12419349
'The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the biotin/biotin-binding protein interaction is that it is essentially irreversible under physiological conditions. Desthiobiotin is a biotin analogue that binds less tightly to biotin-binding proteins and is easily displaced by
Identification of proteins containing cysteine residues that are sensitive to oxidation by hydrogen peroxide at neutral pH.
Journal:Anal Biochem
PubMed ID:10906242
'A procedure for detecting proteins that contain H(2)O(2)-sensitive cysteine (or selenocysteine) residues was developed as a means with which to study protein oxidation by H(2)O(2) in cells. The procedure is based on the facts that H(2)O(2) and biotin-conjugated iodoacetamide (BIAM) selectively and competitively react with cysteine residues that exhibit a
Presenilin-1 affects trafficking and processing of betaAPP and is targeted in a complex with nicastrin to the plasma membrane.
Journal:J Cell Biol
PubMed ID:12147673
'Amyloid beta-peptide (Abeta) is generated by the consecutive cleavages of beta- and gamma-secretase. The intramembraneous gamma-secretase cleavage critically depends on the activity of presenilins (PS1 and PS2). Although there is evidence that PSs are aspartyl proteases with gamma-secretase activity, it remains controversial whether their subcellular localization overlaps with the cellular
Cytoplasmic dynein regulation by subunit heterogeneity and its role in apical transport.
Journal:J Cell Biol
PubMed ID:11425878
'Despite the existence of multiple subunit isoforms for the microtubule motor cytoplasmic dynein, it has not yet been directly shown that dynein complexes with different compositions exhibit different properties. The 14-kD dynein light chain Tctex-1, but not its homologue RP3, binds directly to rhodopsin''s cytoplasmic COOH-terminal tail, which encodes an
Evidence for allosteric linkage between exosites 1 and 2 of thrombin.
Journal:J Biol Chem
PubMed ID:9325262
'Investigations to date have demonstrated that ligand binding to exosites 1 or 2 on thrombin produces conformational changes at the active site. In this study, we directly compared the effect of ligand binding to exosites 1 and 2 on the structure and function of the active site of thrombin and