链霉素亲和素琼脂糖以 10 ml 50% 的浆液形式提供,其中含有 5 ml 压缩包装的凝胶床,溶于 0.01 M 磷酸钠 (pH 7.2)、0.15 M NaCl 和 0.02% 叠氮化钠。在 4°C 下储存。
常见问题解答 (FAQ)
How many biotin binding-sites are there per streptavidin molecule?
Streptavidin is a protein composed of four identical subunits, each containing a high affinity binding site for biotin (K-D = 10 -15 M) . Streptavidin has the same biotin binding properties as avidin, but it has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low non-specific binding.