Streptavidin Agarose is supplied as 10 ml of a 50% slurry containing 5 ml of packed gel bed in 0.01 M sodium phosphate (pH 7.2), 0.15 M NaCl, and 0.02% sodium azide. Store at 4°C.
常见问题解答 (FAQ)
How many biotin binding-sites are there per streptavidin molecule?
Streptavidin is a protein composed of four identical subunits, each containing a high affinity binding site for biotin (K-D = 10 -15 M) . Streptavidin has the same biotin binding properties as avidin, but it has a low isoelectric point (pI=5) and no carbohydrate groups, resulting in low non-specific binding.